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XL GenDNA Extraction Module
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C02010014Auto MagMeDIP kit
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false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'xl-gendna-extraction-module-6-rxns', 'meta_title' => 'XL GenDNA Extraction Module', 'meta_keywords' => '', 'meta_description' => 'XL GenDNA Extraction Module', 'modified' => '2019-04-11 14:22:10', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1880', 'antibody_id' => null, 'name' => 'MagMeDIP Kit', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/magmedip-kit-manual-C02010020-21.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p> <h3><span>Features</span></h3> <ul> <li>Starting DNA amount: <strong>10 ng – 1 µg</strong></li> <li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li> <li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li> <li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li> <li><strong>High reproducibility</strong> between replicates and repetitive experiments</li> <li>Compatible with <strong>all species </strong></li> </ul> <p> </p> <div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div> <div class="small-12 medium-8 large-8 columns"> <h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> <p></p> <p></p> <p></p> <div class="row"> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> <div class="small-12 medium-8 large-8 columns"><br /> <p></p> </div> </div> <h3></h3>', 'label1' => 'MagMeDIP workflow', 'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p> <h3><span>How it works</span></h3> <p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP’d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'MeDIP-qPCR', 'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode’s IP-Star Compact Automated System (please refer to the kit manual).</p> <ul> <li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li> <li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li> <li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li> <li><strong>Highly validated protocol</strong></li> <li>Automated protocol supplied</li> </ul> <center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center> <p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p> <p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 µg or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'MeDIP-seq', 'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p> <ul style="list-style-type: circle;"> <li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 μg). It can be a home-made solution or a commercial one.</li> <li>Choose the indexing system that fits your needs considering the following features:</li> <ul> <ul> <ul> <li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li> <li>Number of barcodes</li> <li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li> <li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li> </ul> </ul> </ul> <li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li> </ul> <p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p> <ul style="list-style-type: circle;"> <li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li> </ul> <ul style="list-style-type: circle;"> <li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li> </ul> <h3><span>MeDIP-seq workflow</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center> <h3><span>Example of results</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1μg – 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency >98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 μg (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p> <p></p> <ul> <ul> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </ul> </ul>', 'format' => '48 rxns (IP)', 'catalog_number' => 'C02010021', 'old_catalog_number' => 'mc-magme-048', 'sf_code' => 'C02010021-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '760', 'price_USD' => '770', 'price_GBP' => '695', 'price_JPY' => '124525', 'price_CNY' => '', 'price_AUD' => '1925', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'magmedip-kit-x48-48-rxns', 'meta_title' => 'MagMeDIP Kit for efficient immunoprecipitation of methylated DNA | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Perform Methylated DNA Immunoprecipitation (MeDIP) to estimate DNA methylation status of your sample using highly specific 5-mC antibody. This kit allows the preparation of cfMeDIP-seq libraries.', 'modified' => '2024-12-04 16:52:47', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. 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12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/magmedip-kit-x48-48-rxns"><img src="/img/product/kits/C02010021-magmedip-qpcr.jpg" alt="MagMeDIP qPCR Kit box" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1880" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1880" id="CartAdd/1880Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1880" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>MagMeDIP Kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('MagMeDIP Kit', 'C02010021', '770', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('MagMeDIP Kit', 'C02010021', '770', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="magmedip-kit-x48-48-rxns" data-reveal-id="cartModal-1880" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">MagMeDIP qPCR kit</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/auto-magmedip-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010014</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Auto MagMeDIP kit</h6> </div> </div> </li> ' $related = array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. 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true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'xl-gendna-extraction-module-6-rxns', 'meta_title' => 'XL GenDNA Extraction Module', 'meta_keywords' => '', 'meta_description' => 'XL GenDNA Extraction Module', 'modified' => '2019-04-11 14:22:10', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1880', 'antibody_id' => null, 'name' => 'MagMeDIP Kit', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/magmedip-kit-manual-C02010020-21.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p> <h3><span>Features</span></h3> <ul> <li>Starting DNA amount: <strong>10 ng – 1 µg</strong></li> <li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li> <li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li> <li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li> <li><strong>High reproducibility</strong> between replicates and repetitive experiments</li> <li>Compatible with <strong>all species </strong></li> </ul> <p> </p> <div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div> <div class="small-12 medium-8 large-8 columns"> <h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> <p></p> <p></p> <p></p> <div class="row"> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> <div class="small-12 medium-8 large-8 columns"><br /> <p></p> </div> </div> <h3></h3>', 'label1' => 'MagMeDIP workflow', 'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p> <h3><span>How it works</span></h3> <p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP’d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'MeDIP-qPCR', 'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode’s IP-Star Compact Automated System (please refer to the kit manual).</p> <ul> <li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li> <li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li> <li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li> <li><strong>Highly validated protocol</strong></li> <li>Automated protocol supplied</li> </ul> <center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center> <p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p> <p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 µg or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'MeDIP-seq', 'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p> <ul style="list-style-type: circle;"> <li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 μg). It can be a home-made solution or a commercial one.</li> <li>Choose the indexing system that fits your needs considering the following features:</li> <ul> <ul> <ul> <li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li> <li>Number of barcodes</li> <li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li> <li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li> </ul> </ul> </ul> <li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li> </ul> <p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p> <ul style="list-style-type: circle;"> <li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li> </ul> <ul style="list-style-type: circle;"> <li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li> </ul> <h3><span>MeDIP-seq workflow</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center> <h3><span>Example of results</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1μg – 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency >98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 μg (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p> <p></p> <ul> <ul> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </ul> </ul>', 'format' => '48 rxns (IP)', 'catalog_number' => 'C02010021', 'old_catalog_number' => 'mc-magme-048', 'sf_code' => 'C02010021-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '760', 'price_USD' => '770', 'price_GBP' => '695', 'price_JPY' => '124525', 'price_CNY' => '', 'price_AUD' => '1925', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'magmedip-kit-x48-48-rxns', 'meta_title' => 'MagMeDIP Kit for efficient immunoprecipitation of methylated DNA | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Perform Methylated DNA Immunoprecipitation (MeDIP) to estimate DNA methylation status of your sample using highly specific 5-mC antibody. This kit allows the preparation of cfMeDIP-seq libraries.', 'modified' => '2024-12-04 16:52:47', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. DNA has been isolated by using DNA Isolation Buffer (DIB).</em></p> <p></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '48 rxns', 'catalog_number' => 'C02010014', 'old_catalog_number' => '', 'sf_code' => 'C02010014-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '', 'price_USD' => '', 'price_GBP' => '', 'price_JPY' => '', 'price_CNY' => '', 'price_AUD' => '', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-magmedip-kit-x48-48-rxns', 'meta_title' => 'Auto MagMeDIP qPCR Kit x48', 'meta_keywords' => '', 'meta_description' => 'Auto MagMeDIP qPCR Kit x48', 'modified' => '2023-03-20 12:50:08', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 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12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/magmedip-kit-x48-48-rxns"><img src="/img/product/kits/C02010021-magmedip-qpcr.jpg" alt="MagMeDIP qPCR Kit box" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1880" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1880" 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<div class="small-12 columns" > <h6 style="height:60px">Auto MagMeDIP kit</h6> </div> </div> </li> ' $related = array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. DNA has been isolated by using DNA Isolation Buffer (DIB).</em></p> <p></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '48 rxns', 'catalog_number' => 'C02010014', 'old_catalog_number' => '', 'sf_code' => 'C02010014-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '', 'price_USD' => '', 'price_GBP' => '', 'price_JPY' => '', 'price_CNY' => '', 'price_AUD' => '', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-magmedip-kit-x48-48-rxns', 'meta_title' => 'Auto MagMeDIP qPCR Kit x48', 'meta_keywords' => '', 'meta_description' => 'Auto MagMeDIP qPCR Kit x48', 'modified' => '2023-03-20 12:50:08', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '4519', 'product_id' => '1922', 'related_id' => '2945' ), 'Image' => array( (int) 0 => array( 'id' => '1778', 'name' => 'product/kits/methyl-kit-icon.png', 'alt' => 'Methylation kit icon', 'modified' => '2019-04-23 15:17:01', 'created' => '2018-03-15 15:52:12', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(mc-magme-003)</span></small>' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '111', 'name' => 'XL-GenDNA Extraction Module', 'description' => '<p>This module has been designed for the extraction of 60 µg of DNA from culture cells.</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/XL-GenDNA_Extraction_Module_manual.pdf', 'slug' => 'xl-gendna-extraction-module-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-02 14:42:07', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '992', 'product_id' => '1922', 'document_id' => '111' ) ) $sds = array( 'id' => '292', 'name' => 'XL GenDNA Extraction Module SDS ES es', 'language' => 'es', 'url' => 'files/SDS/XL_GenDNA/KIT-C03030020-XL_GenDNA_Extraction_Module-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 11:52:50', 'created' => '2020-06-09 11:52:50', 'ProductsSafetySheet' => array( 'id' => '539', 'product_id' => '1922', 'safety_sheet_id' => '292' ) )CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1055 ReflectionMethod::invokeArgs() - [internal], line ?? 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true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'xl-gendna-extraction-module-6-rxns', 'meta_title' => 'XL GenDNA Extraction Module', 'meta_keywords' => '', 'meta_description' => 'XL GenDNA Extraction Module', 'modified' => '2019-04-11 14:22:10', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1880', 'antibody_id' => null, 'name' => 'MagMeDIP Kit', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/magmedip-kit-manual-C02010020-21.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p> <h3><span>Features</span></h3> <ul> <li>Starting DNA amount: <strong>10 ng – 1 µg</strong></li> <li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li> <li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li> <li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li> <li><strong>High reproducibility</strong> between replicates and repetitive experiments</li> <li>Compatible with <strong>all species </strong></li> </ul> <p> </p> <div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div> <div class="small-12 medium-8 large-8 columns"> <h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> <p></p> <p></p> <p></p> <div class="row"> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> <div class="small-12 medium-8 large-8 columns"><br /> <p></p> </div> </div> <h3></h3>', 'label1' => 'MagMeDIP workflow', 'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p> <h3><span>How it works</span></h3> <p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP’d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'MeDIP-qPCR', 'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode’s IP-Star Compact Automated System (please refer to the kit manual).</p> <ul> <li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li> <li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li> <li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li> <li><strong>Highly validated protocol</strong></li> <li>Automated protocol supplied</li> </ul> <center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center> <p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p> <p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 µg or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'MeDIP-seq', 'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p> <ul style="list-style-type: circle;"> <li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 μg). It can be a home-made solution or a commercial one.</li> <li>Choose the indexing system that fits your needs considering the following features:</li> <ul> <ul> <ul> <li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li> <li>Number of barcodes</li> <li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li> <li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li> </ul> </ul> </ul> <li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li> </ul> <p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p> <ul style="list-style-type: circle;"> <li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li> </ul> <ul style="list-style-type: circle;"> <li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li> </ul> <h3><span>MeDIP-seq workflow</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center> <h3><span>Example of results</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1μg – 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency >98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 μg (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p> <p></p> <ul> <ul> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </ul> </ul>', 'format' => '48 rxns (IP)', 'catalog_number' => 'C02010021', 'old_catalog_number' => 'mc-magme-048', 'sf_code' => 'C02010021-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '760', 'price_USD' => '770', 'price_GBP' => '695', 'price_JPY' => '124525', 'price_CNY' => '', 'price_AUD' => '1925', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'magmedip-kit-x48-48-rxns', 'meta_title' => 'MagMeDIP Kit for efficient immunoprecipitation of methylated DNA | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Perform Methylated DNA Immunoprecipitation (MeDIP) to estimate DNA methylation status of your sample using highly specific 5-mC antibody. This kit allows the preparation of cfMeDIP-seq libraries.', 'modified' => '2024-12-04 16:52:47', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. 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12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/magmedip-kit-x48-48-rxns"><img src="/img/product/kits/C02010021-magmedip-qpcr.jpg" alt="MagMeDIP qPCR Kit box" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1880" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1880" 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type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="magmedip-kit-x48-48-rxns" data-reveal-id="cartModal-1880" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">MagMeDIP qPCR kit</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/auto-magmedip-kit-x48-48-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010014</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Auto MagMeDIP kit</h6> </div> </div> </li> ' $related = array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). 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true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'xl-gendna-extraction-module-6-rxns', 'meta_title' => 'XL GenDNA Extraction Module', 'meta_keywords' => '', 'meta_description' => 'XL GenDNA Extraction Module', 'modified' => '2019-04-11 14:22:10', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1880', 'antibody_id' => null, 'name' => 'MagMeDIP Kit', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/magmedip-kit-manual-C02010020-21.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Perform <strong>MeDIP</strong> (<strong>Me</strong>thylated <strong>D</strong>NA <strong>I</strong>mmuno<strong>p</strong>recipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-methylcytosine antibody. Our MagMeDIP kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.</p> <h3><span>Features</span></h3> <ul> <li>Starting DNA amount: <strong>10 ng – 1 µg</strong></li> <li>Content: <strong>all reagents included</strong> for DNA extraction, immunoprecipitation (including the 5-mC antibody, spike-in controls and their corresponding qPCR primer pairs) as well as DNA isolation after IP.</li> <li>Application: <strong>qPCR</strong> and <strong>NGS</strong></li> <li>Robust method, <strong>superior enrichment</strong>, and easy-to-use protocol</li> <li><strong>High reproducibility</strong> between replicates and repetitive experiments</li> <li>Compatible with <strong>all species </strong></li> </ul> <p> </p> <div class="small-12 medium-4 large-4 columns"><center></center><center></center><center></center><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" alt="Click here to read more about MeDIP " caption="false" width="80%" /></a></center></div> <div class="small-12 medium-8 large-8 columns"> <h3 style="text-align: justify;">Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline" style="text-align: justify;">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> <p></p> <p></p> <p></p> <div class="row"> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> <div class="small-12 medium-8 large-8 columns"><br /> <p></p> </div> </div> <h3></h3>', 'label1' => 'MagMeDIP workflow', 'info1' => '<p>DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP Kit takes advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be thereafter directly analyzed by qPCR or Next-Generation Sequencing (NGS).</p> <h3><span>How it works</span></h3> <p>In brief, after the cell collection and lysis, the genomic DNA is extracted, sheared, and then denatured. In the next step the antibody directed against 5 methylcytosine and antibody binding beads are used for immunoselection and immunoprecipitation of methylated DNA fragments. Then, the IP’d methylated DNA is isolated and can be used for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <center><img src="https://www.diagenode.com/img/product/kits/MagMeDIP-workflow.png" width="70%" alt="5-methylcytosine" caption="false" /></center> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'MeDIP-qPCR', 'info2' => '<p>The kit MagMeDIP contains all reagents necessary for a complete MeDIP-qPCR workflow. Two MagMeDIP protocols have been validated: for manual processing as well as for automated processing, using the Diagenode’s IP-Star Compact Automated System (please refer to the kit manual).</p> <ul> <li><strong>Complete kit</strong> including DNA extraction module, IP antibody and reagents, DNA isolation buffer</li> <li><strong>Quality control of the IP:</strong> due to methylated and unmethylated DNA spike-in controls and their associated qPCR primers</li> <li><strong>Easy to use</strong> with user-friendly magnetic beads and rack</li> <li><strong>Highly validated protocol</strong></li> <li>Automated protocol supplied</li> </ul> <center><img src="https://www.diagenode.com/img/product/kits/fig1-magmedipkit.png" width="85%" alt="Methylated DNA Immunoprecipitation" caption="false" /></center> <p style="font-size: 0.9em;"><em><strong>Figure 1.</strong> Immunoprecipitation results obtained with Diagenode MagMeDIP Kit</em></p> <p style="font-size: 0.9em;">MeDIP assays were performed manually using 1 µg or 50 ng gDNA from blood cells with the MagMeDIP kit (Diagenode). The IP was performed with the Methylated and Unmethylated spike-in controls included in the kit, together with the human DNA samples. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs included in this kit.</p> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => 'MeDIP-seq', 'info3' => '<p>For DNA methylation analysis on the whole genome, MagMeDIP kit can be coupled with Next-Generation Sequencing. To perform MeDIP-sequencing we recommend the following strategy:</p> <ul style="list-style-type: circle;"> <li>Choose a library preparation solution which is compatible with the starting amount of DNA you are planning to use (from 10 ng to 1 μg). It can be a home-made solution or a commercial one.</li> <li>Choose the indexing system that fits your needs considering the following features:</li> <ul> <ul> <ul> <li>Single-indexing, combinatorial dual-indexing or unique dual-indexing</li> <li>Number of barcodes</li> <li>Full-length adaptors containing the barcodes or barcoding at the final amplification step</li> <li>Presence / absence of Unique Molecular Identifiers (for PCR duplicates removal)</li> </ul> </ul> </ul> <li>Standard library preparation protocols are compatible with double-stranded DNA only, therefore the first steps of the library preparation (end repair, A-tailing, adaptor ligation and clean-up) will have to be performed on sheared DNA, before the IP.</li> </ul> <p style="padding-left: 30px;"><strong>CAUTION:</strong> As the immunoprecipitation step occurs at the middle of the library preparation workflow, single-tube solutions for library preparation are usually not compatible with MeDIP-sequencing.</p> <ul style="list-style-type: circle;"> <li>For DNA isolation after the IP, we recommend using the <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24" title="IPure kit v2">IPure kit v2</a> (available separately, Cat. No. C03010014) instead of DNA isolation Buffer.</li> </ul> <ul style="list-style-type: circle;"> <li>Perform library amplification after the DNA isolation following the standard protocol of the chosen library preparation solution.</li> </ul> <h3><span>MeDIP-seq workflow</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/MeDIP-seq-workflow.png" width="110%" alt="MagMeDIP qPCR Kit x10 workflow" caption="false" /></center> <h3><span>Example of results</span></h3> <center><img src="https://www.diagenode.com/img/product/kits/medip-specificity.png" alt="MagMeDIP qPCR Kit Result" caption="false" width="951" height="488" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 1. qPCR analysis of external spike-in DNA controls (methylated and unmethylated) after IP.</strong> Samples were prepared using 1μg – 100ng -10ng sheared human gDNA with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-saturation-analysis.png" alt=" MagMeDIP kit " caption="false" width="951" height="461" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 2. Saturation analysis.</strong> Clean reads were aligned to the human genome (hg19) using Burrows-Wheeler aligner (BWA) algorithm after which duplicated and unmapped reads were removed resulting in a mapping efficiency >98% for all samples. Quality and validity check of the mapped MeDIP-seq data was performed using MEDIPS R package. Saturation plots show that all sets of reads have sufficient complexity and depth to saturate the coverage profile of the reference genome and that this is reproducible between replicates and repetitive experiments (data shown for 50 ng gDNA input: left panel = replicate a, right panel = replicate b).</p> <p></p> <p></p> <center><img src="https://www.diagenode.com/img/product/kits/medip-libraries-prep.png" alt="MagMeDIP x10 " caption="false" width="951" height="708" /></center> <p></p> <p style="font-size: 0.9em;"><strong>Figure 3. Sequencing profiles of MeDIP-seq libraries prepared from different starting amounts of sheared gDNA on the positive and negative methylated control regions.</strong> MeDIP-seq libraries were prepared from decreasing starting amounts of gDNA (1 μg (green), 50 ng (red), and 10ng (blue)) originating from human blood with the MagMeDIP kit (Diagenode) and a commercially available library prep kit. DNA isolation after IP has been performed with IPure kit V2 (Diagenode). IP and corresponding INPUT samples were sequenced on Illumina NovaSeq SP with 2x50 PE reads. The reads were mapped to the human genome (hg19) with bwa and the alignments were loaded into IGV (the tracks use an identical scale). The top IGV figure shows the TSH2B (also known as H2BC1) gene (marked by blue boxes in the bottom track) and its surroundings. The TSH2B gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene. The bottom IGV figure shows the GADPH locus (marked by blue boxes in the bottom track) and its surroundings. The GADPH gene is a highly active transcription region and should not be methylated, resulting in no reads accumulation following MeDIP-seq experiment.</p> <p></p> <ul> <ul> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </ul> </ul>', 'format' => '48 rxns (IP)', 'catalog_number' => 'C02010021', 'old_catalog_number' => 'mc-magme-048', 'sf_code' => 'C02010021-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '760', 'price_USD' => '770', 'price_GBP' => '695', 'price_JPY' => '124525', 'price_CNY' => '', 'price_AUD' => '1925', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'magmedip-kit-x48-48-rxns', 'meta_title' => 'MagMeDIP Kit for efficient immunoprecipitation of methylated DNA | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Perform Methylated DNA Immunoprecipitation (MeDIP) to estimate DNA methylation status of your sample using highly specific 5-mC antibody. This kit allows the preparation of cfMeDIP-seq libraries.', 'modified' => '2024-12-04 16:52:47', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. 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<div class="small-12 columns" > <h6 style="height:60px">Auto MagMeDIP kit</h6> </div> </div> </li> ' $related = array( 'id' => '2945', 'antibody_id' => null, 'name' => 'Auto MagMeDIP qPCR Kit - ordering reference: C02010021', 'description' => '<p><span></span>The reference C02010014 has been replaced by <a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns">C02010021</a><span>. </span> </p> <p><span>Perform </span><strong>MeDIP</strong><span><span> </span>(Methylated DNA Immunoprecipitation) <span>on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> </span>followed by<span> </span></span><strong>qPCR</strong><span><span> </span>to estimate DNA methylation status of your sample using </span><span>5-methylcytosine</span><span><span> </span>antibody. Our kit contains high quality reagents to get the h</span><span>ighest enrichment of methylated DNA with an optimized user-friendly protocol.</span></p> <p>Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit" target="_blank">SX-8G IP-Star® Automated System</a> enabling highly reproducible results and allowing for high throughput assays.</p> <h3><span>Characteristics</span></h3> <ul> <li>Generate highly consistent results with internal controls in 24h</li> <li>Minimize error with many reagents in 1 tube</li> <li>Optimized purification (DIB - DNA isolation buffer)</li> <li>Allows direct correlation between IP’d material & methylation status</li> </ul> <p style="text-align: center;"><img src="https://www.diagenode.com/img/product/kits/magmedip-kit-validated-using-bioruptor.jpg" alt="MagMeDIP kit validated using Bioruptor" /></p> <p><strong><em>Figure 1.</em></strong><em><span> </span><strong>IP results obtained with Diagenode Auto MagMeDIP qPCR Kit.</strong><span> </span>MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.</em></p> <p style="text-align: center;"><em><img src="https://www.diagenode.com/img/product/kits/AutomatedMeDIP_9h.png" alt="" width="678" height="365" /></em></p> <p style="text-align: justify;"><em><strong>Figure<span> </span>2. Automated MeDIP (9h). </strong>IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. 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