ChIPmentation™

最適なChIP-seqのためのChIPmentationシステムの利点

• 高品質のシーケンスデータ
• 低入力サンプルで可能なChIP-seq
• 簡単で高速なプロトコール
• シーケンシングアダプターダイマーの排除
• IP-Star®で自動化、標準化をサポートして時間を節約。

• ChIPmentationワークフロー-更に詳しく
  

  In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.

完全なキット：

• µChIPmentation for Histones
  The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.
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• ChIPmentation Kit for Histones
  ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.
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モジュール：

• TAG Kit for ChIPmentation
  The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.
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Primer indexes for tagmented librairies:

• 24 SI for ChIPmentation
  The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.
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• 24 SI for Tagmented libraries
  The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.
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• 24 UDI for Tagmented libraries - Set I
  The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.
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• 24 UDI for Tagmented libraries - Set II
  The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.
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別途販売製品：

• Tagmentase (Tn5 transposase) - loaded
  Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.
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• Tagmentase (Tn5 transposase) - unloaded
  Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.
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• Tagmentase Dilution Buffer
  Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.
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• Tagmentation Buffer (1x)
  Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.
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