Chromatin studies

ChIPmentation™

ChIPmentation for high-quality and fast
ChIP-seq library preparation

Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol. Two versions of complete protocol have been validated: a manual procedure and an automated protocol on the IP-Star Compact Automated System. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.

New! Additionally, the TAG Kit for ChIPmentation - the module for tagmentation compatible with any ChIP protocol is now available.

Benefits of the ChIPmentation system for optimal ChIP-seq

Ensure high quality sequencing data
Save one full day from standard protocols in generating ChIP-seq libraries
Enjoy an easier protocol
Take advantage of the flexibility: use our complete solution ChIPmentation for Histones or your own ChIP method combined with our TAG Kit for ChIPmentation and 24 SI for ChIPmentation
Benefit from the elimination of sequencing adaptor dimers
Automate on the IP-Star® to support standardization and save time

ChIPmentation workflow - Read more

In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.

Complete kits:

Auto ChIPmentation Kit for Histones
ChIPmentation, incorporates automation with an ligation-free protocol that integrates chromatin immunoprecipitation with NGS library preparation.
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ChIPmentation Kit for Histones
ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.
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Modules:

TAG Kit for ChIPmentation
The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.
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24 SI for ChIPmentation
The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.
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产品

目录号	产品	格式	价格
C01011011	µChIPmentation for Histones The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries. T...	24 rxns	$2,400.00
C01011032	SI for Tagmented libraries The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries ar...	24 SI	$190.00
C01011000	Auto ChIPmentation Kit for Histones Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ...	24 rxns
C01011010	ChIPmentation Kit for Histones Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ...	24 rxns
C01011030	TAG Kit for ChIPmentation Contact us Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fra...	24 rxns	$1,050.00