Rsf1 monoclonal antibody

Catalog Number

Format

Price

C15200041
(MAb-041-050)

50 µg/39 µl

$390.00
Bulk order

Alternative names:HBXAP, RSF-1, XAP8, p325

Monoclonal antibody raised in mouse using a recombinant human RSF1 (remodeling and spacing factor 1) protein.

Lot	001
Concentration	1.3 µg/µl
Species reactivity	Human
Type	Monoclonal
Purity	Protein G purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:500	Fig 1
Immunofluorescence	1:100 - 1:500	Ref 1
Immunoprecipitation	0.3 μg	Ref 1

Validation Data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 μg) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right

実験手法

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

IF
Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more

IP
Immunoprecipitation Read more

資料
Datasheet hRSF1 MAb-041-050 DATASHEET
Datasheet description Download

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
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 (Notice): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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 (Notice): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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<th>References</th>
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<td>1:500</td>
<td>Fig 1</td>
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<tr>
<td>Immunofluorescence</td>
<td>1:100 - 1:500</td>
<td>Ref 1</td>
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<tr>
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<td>Ref 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1 </strong><br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right </small></p>
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			'description' => 'RSF1 (UniProtKB/Swiss-Prot entry Q96T23) is required for assembly of regular nucleosome arrays by the RSF chromatin remodelling complex. RSF1 facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappaB transcription activation. RSF1 represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain.
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<thead>
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<th>Suggested dilution</th>
<th>References</th>
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<td>Fig 1</td>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1 </strong><br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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	'name' => 'Rsf1 Antibody SDS BE fr',
	'language' => 'fr',
	'url' => 'files/SDS/Rsf1/SDS-C15200041-Rsf1_Antibody-BE-fr-GHS_1_0.pdf',
	'countries' => 'BE',
	'modified' => '2023-01-10 15:45:22',
	'created' => '2023-01-10 15:45:22',
	'ProductsSafetySheet' => array(
		'id' => '4993',
		'product_id' => '1976',
		'safety_sheet_id' => '3041'
	)
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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