m1I Antibody (sample size)

Catalog Number

Format

Price

C15410366-10

10 µg

$120.00
Bulk order

Other format

C15410366

50 µg

Full name: 1-methylinosine polyclonal antibody

Polyclonal antibody raised in rabbit against 1-methylinosine (m1I) conjugated to BSA.

Lot	A2972-0010
Concentration	2.1 µg/µl
Species reactivity	Human, mouse, other (wide range): positive
Type	Polyclonal
Purity	Protein A purified polyclonal antibody
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	PBS containing 0.05% azide
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Validation data

Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I
To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.
Target Description
1-methylinosine (m1I) is a modified base which is mainly present in tRNAs.
実験手法
DB
Dot blotting Read more

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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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<div class="small-6 columns">
<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15410366_dblot.png" alt="m1I Antibody Dot Blot validation" /></center></div>
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<p><strong>Figure 1. Dot blot analysis using the Diagenode antibody directed against m1I</strong><br />To demonstrate the specificity of the Diagenode antibody against m1I (cat. No. C15410366), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted of 1:400 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 1-methylinosine modification.</p>
</div>
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m1I polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against 1-methylinosine (m1I) conjugated to BSA.	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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