LSD1 polyclonal antibody

Catalog Number

Format

Price

C15410125
(pAb-125-050)

50 µg/75 µl

$370.00
Bulk order

Alternative names: BHC110, AOF2, EC1, KDM1

Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.

Lot	A56-001
Concentration	0.67 µg/µl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100	Fig 1
Western Blotting	1:1,000	Fig 2, Fig 3
Immunoprecipitation	2 μg/IP	Fig 3

Validation Data

Figure 1 Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.

Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1
Western blot was performed using respectively 150 μg (lane 1) or 50 μg (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left.

Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1
293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 μg of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.

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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><span>Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.</span></p>',
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410125_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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		'name' => 'LSD1 polyclonal antibody',
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		'lot' => 'A56-001 ',
		'concentration' => '0.67 &micro;g/&micro;l',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
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		'classification' => 'Classic',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, Fig 3</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>2 &mu;g/IP</td>
<td>Fig 3</td>
</tr>
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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			'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.</span></p>',
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<p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human LSD1 (Cat. No. pAb-125-050). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500. </small></p>
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<p><small><strong> Figure 2 Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using respectively 150 &mu;g (lane 1) or 50 &mu;g (lane 2) nuclear extracts from HeLa cells and the Diagenode antibody against LSD1 (Cat. No. pAb-125-050) diluted 1:1,000. The location of the protein of interest is indicated on the left. </small></p>
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<p><small><strong> Figure 3 Immunoprecipitation and Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the Diagenode antibody against human LSD1 (Cat. No. pAb-125-050), diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 &mu;g of the Diagenode antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above. </small></p>
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
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Datasheet LSD1 pAb-125-050 DATASHEET Datasheet description	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

LSD1 Antibody SDS GB en	Download
LSD1 Antibody SDS US en	Download
LSD1 Antibody SDS DE de	Download
LSD1 Antibody SDS BE nl	Download
LSD1 Antibody SDS FR fr	Download
LSD1 Antibody SDS BE fr	Download
LSD1 Antibody SDS ES es	Download
LSD1 Antibody SDS JP ja	Download

LSD1 polyclonal antibody

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