ELAVL3 polyclonal antibody

Catalog Number

Format

Price

C15410348

100 µg

$390.00
Bulk order

Polyclonal antibody raised in rabbit against human ELAVL3 (ELAV Like RNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.

Lot	001
Concentration	1 μg/μl
Species reactivity	Human, mouse: positive. Other species: not tested.
Type	Polyclonal
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C.
Storage Buffer	PBS containing 50% glycerol, does not contain a preservative.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP	15 µg per 6x10⁶ cells	Fig 1
Western blotting	1:1,000	Fig 2
IP	5 µg per 2.5x10⁶ cells	Fig 3

Validation data

Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3
Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 µg of the Diagenode antibody against ELAVL3 (Cat. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3
Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (Cat. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3
Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 µg of the Diagenode antibody against ELAVL3 (Cat. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.

資料
ELAVL3 polyclonal antibody datasheet DATASHEET
Polyclonal antibody raised in rabbit against human ELAVL3 (ELAV Like RNA Binding Protein 3), usin... Download

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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<td>Fig 1</td>
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<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<td>Fig 1</td>
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<td>Fig 2</td>
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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 (Notice): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<td>Western blotting</td>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>RIP</td>
<td>15 &micro;g per 6x10<sup>6</sup> cells</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>IP</td>
<td>5 &micro;g per 2.5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<li>Batch-specific data is available on the website</li>
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ELAVL3 polyclonal antibody

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