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6X His epitope tag monoclonal antibody
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| Lot | 001 |
|---|---|
| Concentration | 1.0 μg/μl |
| Species reactivity | |
| Type | Monoclonal |
| Purity | Affinity purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:75,000 | |
| Western Blotting | 1:1,000 | |
| Immunoprecipitation | 1:200 | Fig 1, 2 |
- Validation Data

Figure 1. 6X His Tag antibody ELISA results
ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000.
Figure 2. 6X His Tag antibody western blot results
Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT.
Figure 3. 6X His Tag antibody western blot results
Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. - 実験手法
- 資料
Datasheet 6X His epitope tag C15200208 DATASHEET
Datasheet descriptionDownload - Safety sheets
SDS C15200208 6X His epitope tag Antibody US en Download SDS C15200208 6X His epitope tag Antibody GB en Download SDS C15200208 6X His epitope tag Antibody FR fr Download SDS C15200208 6X His epitope tag Antibody BE fr Download SDS C15200208 6X His epitope tag Antibody ES es Download SDS C15200208 6X His epitope tag Antibody JP ja Download SDS C15200208 6X His epitope tag Antibody DE de Download SDS C15200208 6X His epitope tag Antibody BE nl Download - 出版物

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Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). 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Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). 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(Notice): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context$errorType = self::getErrorType($code);self::logToDatabase( $errorType, $description, $file, $line);return parent::handleError( $errorType, $description, $file, $line, $context);$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2018', 'antibody_id' => '330', 'name' => '6X His epitope tag monoclonal antibody ', 'description' => '<p><span>Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-ELISA.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="319" height="239" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200208', 'old_catalog_number' => '', 'sf_code' => 'C15200208-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '445', 'price_USD' => '460', 'price_GBP' => '405', 'price_JPY' => '72915', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '6x-his-epitope-tag-monoclonal-antibody-classic-100-ug', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'modified' => '2024-01-17 16:00:19', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '330', 'name' => '6X His epitope tag monoclonal antibody - Classic', 'description' => '6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. 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Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). 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(Notice): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context$errorType = self::getErrorType($code);self::logToDatabase( $errorType, $description, $file, $line);return parent::handleError( $errorType, $description, $file, $line, $context);$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2018', 'antibody_id' => '330', 'name' => '6X His epitope tag monoclonal antibody ', 'description' => '<p><span>Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-ELISA.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="319" height="239" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200208', 'old_catalog_number' => '', 'sf_code' => 'C15200208-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '445', 'price_USD' => '460', 'price_GBP' => '405', 'price_JPY' => '72915', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '6x-his-epitope-tag-monoclonal-antibody-classic-100-ug', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'modified' => '2024-01-17 16:00:19', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '330', 'name' => '6X His epitope tag monoclonal antibody - Classic', 'description' => '6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. 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Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). 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(Notice): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context$errorType = self::getErrorType($code);self::logToDatabase( $errorType, $description, $file, $line);return parent::handleError( $errorType, $description, $file, $line, $context);$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2018', 'antibody_id' => '330', 'name' => '6X His epitope tag monoclonal antibody ', 'description' => '<p><span>Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-ELISA.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="319" height="239" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200208', 'old_catalog_number' => '', 'sf_code' => 'C15200208-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '445', 'price_USD' => '460', 'price_GBP' => '405', 'price_JPY' => '72915', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '6x-his-epitope-tag-monoclonal-antibody-classic-100-ug', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'modified' => '2024-01-17 16:00:19', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '330', 'name' => '6X His epitope tag monoclonal antibody - Classic', 'description' => '6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. 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Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200208', 'old_catalog_number' => '', 'sf_code' => 'C15200208-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '445', 'price_USD' => '460', 'price_GBP' => '405', 'price_JPY' => '72915', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '6x-his-epitope-tag-monoclonal-antibody-classic-100-ug', 'meta_title' => '6X His epitope tag monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => '6X His epitope tag monoclonal antibody - Classic', 'modified' => '2024-01-17 16:00:19', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '330', 'name' => '6X His epitope tag monoclonal antibody - Classic', 'description' => '6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. 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