タンパク質分析
タンパク質抽出
Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Diagenode's Bioruptor® deliver high quality protein.
Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).
Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).
タンパク質抽出サービスの推奨・関連製品
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| B01080010 |
Picoruptor 2 sonication device 環境研究、毒物学、ゲノミクスおよびエピゲノミクス、癌研究、幹細胞開発、神経科学、臨床応用、農業、その他多数を含むさまざまな研究分野でのサンプル調製において、最高のプロセスを目指して設計開発されたPicoruptorと共にDiagenodeは、2004年以来断片化に関する専門知識と経験を積み重ねてきました。 ... |
1 unit | ||
| B01020014 |
Bioruptor® Plus sonication device The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate... |
1 unit | ||
| C30010010-50 |
1.5 ml Bioruptor® Plus TPX microtubes TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultra... |
50 pc | $60.00 | |
| B01200045 |
iST cartridge holder for the Picoruptor (PreOmics) The iST cartridge holder has been especially designed for being used with the Picoruptor® sonication device and with the PreOmics iST sample prepar... |
1 pack | $1,750.00 | |
| C20000021 |
Protein Extraction beads Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein... |
20 g | $105.00 | |
| C20000020 |
Protein Extraction kit for Bioruptor® Plus Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein... |
50 rxns | $450.00 | |
| B01201140 |
Tube holder for 1.5 ml tubes - Picoruptor The tube holder for 1.5 ml tubes has been especially designed for being used with the Picoruptor sonication device. It allows for reproducible sonication of ... |
1 unit | $1,900.00 |
関連するプロトコル
| Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico Protein extraction from tissues and cultured cells is the first step for many biochemical and ana... | ダウンロード |
| TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor® The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com... | ダウンロード |
| Activity Test - Procedure for immunoprecipitation of native chromatin proteins Proteins play role in almost all cellular processes, and the study of their activity is crucial f... | ダウンロード |
| Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor® The regular protocol for the extraction of histone requires an acid extraction, making impossible... | ダウンロード |
| Mass-Spectrometry: procedure for shotgun proteomics Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Sh... | ダウンロード |
| Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck ... | ダウンロード |
| iST Sample Preparation with the Bioruptor® The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e... | ダウンロード |