Rat GAPDH promoter +0.3 kb primer pair

Catalog Number

Format

Price

C17031046-50
(pp-1046-050)

50 µl

$60.00

Other format

C17031046-500

500 µl

The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).

Documents
Datasheet Rat GAPDH pp1046 DATASHEET
Datasheet description Download

Publications

How to properly cite our product/service in your work

We strongly recommend using this: Rat GAPDH promoter +0.3 kb primer pair (Hologic Diagenode Cat# C17031046-50). Click here to copy to clipboard.

Using our products or services in your publication? Let us know!

Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.
Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1
During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and prom...

 (Notice): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'product' => array(
		'Product' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	),
	'meta_canonical' => 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
	'fromEmail' => 'dev.diagenode.com'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Rat GAPDH promoter +0.3 kb primer pair'
$meta_title = 'Rat GAPDH promoter +0.3 kb primer pair'
$product = array(
	'Product' => array(
		'id' => '2599',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '50 µl',
		'catalog_number' => 'C17031046-50',
		'old_catalog_number' => 'pp-1046-050',
		'sf_code' => 'C17031046-D001-000014',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '60',
		'price_USD' => '60',
		'price_GBP' => '50',
		'price_JPY' => '9830',
		'price_CNY' => '',
		'price_AUD' => '150',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2015-09-02 11:05:49',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(
		(int) 0 => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		)
	),
	'Group' => array(
		'Group' => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		),
		'Master' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '71',
			'position' => '1',
			'parent_id' => '35',
			'name' => 'Rat',
			'description' => '<p>Primer pairs - Rat</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'primer-pairs-rat',
			'cookies_tag_id' => null,
			'meta_keywords' => 'primer pairs rat ,polymerase chain reaction (qPCR),diagenode',
			'meta_description' => 'Diagenode offers Primer pairs specific to a CpG region of the TSH2B gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR)',
			'meta_title' => 'Primer pairs - Rat | Diagenode',
			'modified' => '2016-03-02 08:36:35',
			'created' => '2015-07-23 15:48:20',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '260',
			'name' => 'Datasheet Rat GAPDH pp1046',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
			'slug' => 'datasheet-rat-gapdh-pp1046',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:43',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3463',
			'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
			'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
			'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
			'date' => '2018-03-02',
			'pmid' => 'http://www.pubmed.gov/29500414',
			'doi' => '10.1038/s41419-018-0396-1',
			'modified' => '2019-02-15 21:21:45',
			'created' => '2019-02-14 15:01:22',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$meta_canonical = 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul'
$fromEmail = 'dev.diagenode.com'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
	(int) 0 => array(
		'id' => '2600',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '500 µl',
		'catalog_number' => 'C17031046-500',
		'old_catalog_number' => 'pp-1046-500',
		'sf_code' => 'C17031046-D001-000015',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '120',
		'price_USD' => '115',
		'price_GBP' => '105',
		'price_JPY' => '19660',
		'price_CNY' => '',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2016-02-19 22:03:21',
		'created' => '2015-06-29 14:08:20',
		'ProductsGroup' => array(
			'id' => '149',
			'product_id' => '2600',
			'group_id' => '138'
		)
	)
)
$pro = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(pp-1046-500)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '260',
	'name' => 'Datasheet Rat GAPDH pp1046',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
	'slug' => 'datasheet-rat-gapdh-pp1046',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:43',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '901',
		'product_id' => '2599',
		'document_id' => '260'
	)
)
$publication = array(
	'id' => '3463',
	'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
	'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
	'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
	'date' => '2018-03-02',
	'pmid' => 'http://www.pubmed.gov/29500414',
	'doi' => '10.1038/s41419-018-0396-1',
	'modified' => '2019-02-15 21:21:45',
	'created' => '2019-02-14 15:01:22',
	'ProductsPublication' => array(
		'id' => '3104',
		'product_id' => '2599',
		'publication_id' => '3463'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/29500414" target="_blank"><i class="fa fa-external-link"></i></a>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'product' => array(
		'Product' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	),
	'meta_canonical' => 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
	'fromEmail' => 'dev.diagenode.com'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Rat GAPDH promoter +0.3 kb primer pair'
$meta_title = 'Rat GAPDH promoter +0.3 kb primer pair'
$product = array(
	'Product' => array(
		'id' => '2599',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '50 µl',
		'catalog_number' => 'C17031046-50',
		'old_catalog_number' => 'pp-1046-050',
		'sf_code' => 'C17031046-D001-000014',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '60',
		'price_USD' => '60',
		'price_GBP' => '50',
		'price_JPY' => '9830',
		'price_CNY' => '',
		'price_AUD' => '150',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2015-09-02 11:05:49',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(
		(int) 0 => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		)
	),
	'Group' => array(
		'Group' => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		),
		'Master' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '71',
			'position' => '1',
			'parent_id' => '35',
			'name' => 'Rat',
			'description' => '<p>Primer pairs - Rat</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'primer-pairs-rat',
			'cookies_tag_id' => null,
			'meta_keywords' => 'primer pairs rat ,polymerase chain reaction (qPCR),diagenode',
			'meta_description' => 'Diagenode offers Primer pairs specific to a CpG region of the TSH2B gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR)',
			'meta_title' => 'Primer pairs - Rat | Diagenode',
			'modified' => '2016-03-02 08:36:35',
			'created' => '2015-07-23 15:48:20',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '260',
			'name' => 'Datasheet Rat GAPDH pp1046',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
			'slug' => 'datasheet-rat-gapdh-pp1046',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:43',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3463',
			'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
			'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
			'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
			'date' => '2018-03-02',
			'pmid' => 'http://www.pubmed.gov/29500414',
			'doi' => '10.1038/s41419-018-0396-1',
			'modified' => '2019-02-15 21:21:45',
			'created' => '2019-02-14 15:01:22',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$meta_canonical = 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul'
$fromEmail = 'dev.diagenode.com'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
	(int) 0 => array(
		'id' => '2600',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '500 µl',
		'catalog_number' => 'C17031046-500',
		'old_catalog_number' => 'pp-1046-500',
		'sf_code' => 'C17031046-D001-000015',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '120',
		'price_USD' => '115',
		'price_GBP' => '105',
		'price_JPY' => '19660',
		'price_CNY' => '',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2016-02-19 22:03:21',
		'created' => '2015-06-29 14:08:20',
		'ProductsGroup' => array(
			'id' => '149',
			'product_id' => '2600',
			'group_id' => '138'
		)
	)
)
$pro = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(pp-1046-500)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '260',
	'name' => 'Datasheet Rat GAPDH pp1046',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
	'slug' => 'datasheet-rat-gapdh-pp1046',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:43',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '901',
		'product_id' => '2599',
		'document_id' => '260'
	)
)
$publication = array(
	'id' => '3463',
	'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
	'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
	'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
	'date' => '2018-03-02',
	'pmid' => 'http://www.pubmed.gov/29500414',
	'doi' => '10.1038/s41419-018-0396-1',
	'modified' => '2019-02-15 21:21:45',
	'created' => '2019-02-14 15:01:22',
	'ProductsPublication' => array(
		'id' => '3104',
		'product_id' => '2599',
		'publication_id' => '3463'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/29500414" target="_blank"><i class="fa fa-external-link"></i></a>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'product' => array(
		'Product' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	),
	'meta_canonical' => 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
	'fromEmail' => 'dev.diagenode.com'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Rat GAPDH promoter +0.3 kb primer pair'
$meta_title = 'Rat GAPDH promoter +0.3 kb primer pair'
$product = array(
	'Product' => array(
		'id' => '2599',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '50 µl',
		'catalog_number' => 'C17031046-50',
		'old_catalog_number' => 'pp-1046-050',
		'sf_code' => 'C17031046-D001-000014',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '60',
		'price_USD' => '60',
		'price_GBP' => '50',
		'price_JPY' => '9830',
		'price_CNY' => '',
		'price_AUD' => '150',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2015-09-02 11:05:49',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(
		(int) 0 => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		)
	),
	'Group' => array(
		'Group' => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		),
		'Master' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '71',
			'position' => '1',
			'parent_id' => '35',
			'name' => 'Rat',
			'description' => '<p>Primer pairs - Rat</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'primer-pairs-rat',
			'cookies_tag_id' => null,
			'meta_keywords' => 'primer pairs rat ,polymerase chain reaction (qPCR),diagenode',
			'meta_description' => 'Diagenode offers Primer pairs specific to a CpG region of the TSH2B gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR)',
			'meta_title' => 'Primer pairs - Rat | Diagenode',
			'modified' => '2016-03-02 08:36:35',
			'created' => '2015-07-23 15:48:20',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '260',
			'name' => 'Datasheet Rat GAPDH pp1046',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
			'slug' => 'datasheet-rat-gapdh-pp1046',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:43',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3463',
			'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
			'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
			'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
			'date' => '2018-03-02',
			'pmid' => 'http://www.pubmed.gov/29500414',
			'doi' => '10.1038/s41419-018-0396-1',
			'modified' => '2019-02-15 21:21:45',
			'created' => '2019-02-14 15:01:22',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$meta_canonical = 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul'
$fromEmail = 'dev.diagenode.com'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
	(int) 0 => array(
		'id' => '2600',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '500 µl',
		'catalog_number' => 'C17031046-500',
		'old_catalog_number' => 'pp-1046-500',
		'sf_code' => 'C17031046-D001-000015',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '120',
		'price_USD' => '115',
		'price_GBP' => '105',
		'price_JPY' => '19660',
		'price_CNY' => '',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2016-02-19 22:03:21',
		'created' => '2015-06-29 14:08:20',
		'ProductsGroup' => array(
			'id' => '149',
			'product_id' => '2600',
			'group_id' => '138'
		)
	)
)
$pro = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(pp-1046-500)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '260',
	'name' => 'Datasheet Rat GAPDH pp1046',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
	'slug' => 'datasheet-rat-gapdh-pp1046',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:43',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '901',
		'product_id' => '2599',
		'document_id' => '260'
	)
)
$publication = array(
	'id' => '3463',
	'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
	'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
	'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
	'date' => '2018-03-02',
	'pmid' => 'http://www.pubmed.gov/29500414',
	'doi' => '10.1038/s41419-018-0396-1',
	'modified' => '2019-02-15 21:21:45',
	'created' => '2019-02-14 15:01:22',
	'ProductsPublication' => array(
		'id' => '3104',
		'product_id' => '2599',
		'publication_id' => '3463'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/29500414" target="_blank"><i class="fa fa-external-link"></i></a>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'product' => array(
		'Product' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Group' => array(
			'Group' => array(
				[maximum depth reached]
			),
			'Master' => array(
				[maximum depth reached]
			),
			'Product' => array(
				[maximum depth reached]
			)
		),
		'Related' => array(),
		'Application' => array(),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	),
	'meta_canonical' => 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
	'fromEmail' => 'dev.diagenode.com'
)
$language = 'en'
$meta_keywords = ''
$meta_description = 'Rat GAPDH promoter +0.3 kb primer pair'
$meta_title = 'Rat GAPDH promoter +0.3 kb primer pair'
$product = array(
	'Product' => array(
		'id' => '2599',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '50 µl',
		'catalog_number' => 'C17031046-50',
		'old_catalog_number' => 'pp-1046-050',
		'sf_code' => 'C17031046-D001-000014',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '60',
		'price_USD' => '60',
		'price_GBP' => '50',
		'price_JPY' => '9830',
		'price_CNY' => '',
		'price_AUD' => '150',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2015-09-02 11:05:49',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(
		(int) 0 => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		)
	),
	'Group' => array(
		'Group' => array(
			'id' => '138',
			'name' => 'C17031046',
			'product_id' => '2599',
			'modified' => '2016-02-19 22:02:31',
			'created' => '2016-02-19 22:02:31'
		),
		'Master' => array(
			'id' => '2599',
			'antibody_id' => null,
			'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 µl',
			'catalog_number' => 'C17031046-50',
			'old_catalog_number' => 'pp-1046-050',
			'sf_code' => 'C17031046-D001-000014',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '60',
			'price_USD' => '60',
			'price_GBP' => '50',
			'price_JPY' => '9830',
			'price_CNY' => '',
			'price_AUD' => '150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-50-ul',
			'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'meta_keywords' => '',
			'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
			'modified' => '2015-09-02 11:05:49',
			'created' => '2015-06-29 14:08:20'
		),
		'Product' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		)
	),
	'Related' => array(),
	'Application' => array(),
	'Category' => array(
		(int) 0 => array(
			'id' => '71',
			'position' => '1',
			'parent_id' => '35',
			'name' => 'Rat',
			'description' => '<p>Primer pairs - Rat</p>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'primer-pairs-rat',
			'cookies_tag_id' => null,
			'meta_keywords' => 'primer pairs rat ,polymerase chain reaction (qPCR),diagenode',
			'meta_description' => 'Diagenode offers Primer pairs specific to a CpG region of the TSH2B gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR)',
			'meta_title' => 'Primer pairs - Rat | Diagenode',
			'modified' => '2016-03-02 08:36:35',
			'created' => '2015-07-23 15:48:20',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '260',
			'name' => 'Datasheet Rat GAPDH pp1046',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
			'slug' => 'datasheet-rat-gapdh-pp1046',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:43',
			'created' => '2015-07-07 11:47:43',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3463',
			'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
			'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
			'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
			'date' => '2018-03-02',
			'pmid' => 'http://www.pubmed.gov/29500414',
			'doi' => '10.1038/s41419-018-0396-1',
			'modified' => '2019-02-15 21:21:45',
			'created' => '2019-02-14 15:01:22',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array()
)
$meta_canonical = 'https://dev.diagenode.com/en/p/rat-gapdh-promoter-0-3-kb-primer-pair-50-ul'
$fromEmail = 'dev.diagenode.com'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
	(int) 0 => array(
		'id' => '2600',
		'antibody_id' => null,
		'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '500 µl',
		'catalog_number' => 'C17031046-500',
		'old_catalog_number' => 'pp-1046-500',
		'sf_code' => 'C17031046-D001-000015',
		'type' => 'FRE',
		'search_order' => '04-undefined',
		'price_EUR' => '120',
		'price_USD' => '115',
		'price_GBP' => '105',
		'price_JPY' => '19660',
		'price_CNY' => '',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
		'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'meta_keywords' => '',
		'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
		'modified' => '2016-02-19 22:03:21',
		'created' => '2015-06-29 14:08:20',
		'ProductsGroup' => array(
			'id' => '149',
			'product_id' => '2600',
			'group_id' => '138'
		)
	)
)
$pro = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ' <span style="color:#CCC">(pp-1046-500)</span>'
$country_code = 'US'
$other_format = array(
	'id' => '2600',
	'antibody_id' => null,
	'name' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'description' => '<p><span>The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '500 µl',
	'catalog_number' => 'C17031046-500',
	'old_catalog_number' => 'pp-1046-500',
	'sf_code' => 'C17031046-D001-000015',
	'type' => 'FRE',
	'search_order' => '04-undefined',
	'price_EUR' => '120',
	'price_USD' => '115',
	'price_GBP' => '105',
	'price_JPY' => '19660',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'rat-gapdh-promoter-0-3-kb-primer-pair-500-ul',
	'meta_title' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'meta_keywords' => '',
	'meta_description' => 'Rat GAPDH promoter +0.3 kb primer pair',
	'modified' => '2016-02-19 22:03:21',
	'created' => '2015-06-29 14:08:20',
	'ProductsGroup' => array(
		'id' => '149',
		'product_id' => '2600',
		'group_id' => '138'
	)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '260',
	'name' => 'Datasheet Rat GAPDH pp1046',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/reagents/primer_pairs/Datasheet_Rat_GAPDH_pp1046.pdf',
	'slug' => 'datasheet-rat-gapdh-pp1046',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:43',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '901',
		'product_id' => '2599',
		'document_id' => '260'
	)
)
$publication = array(
	'id' => '3463',
	'name' => 'Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.',
	'authors' => 'Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1',
	'description' => '<p>During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and promoting the expression of neurogenic bHLH transcription factors. Our studies and others have revealed a link between neuronal differentiation and increase of mitochondrial mass. However, the neuronal regulation of mitochondrial biogenesis has remained largely unexplored. Here, we show that the HDACi, sodium butyrate (NaBt), promotes mitochondrial biogenesis via the NRF-1/Tfam axis in embryonic hippocampal progenitor cells and neuroprogenitor-like PC12-NeuroD6 cells, thereby enhancing their neuronal differentiation competency. Increased mitochondrial DNA replication by several pan-HDACis indicates a common mechanism by which they regulate mitochondrial biogenesis. NaBt also induces coordinates mitochondrial ultrastructural changes and enhanced OXPHOS metabolism, thereby increasing key mitochondrial bioenergetics parameters in neural progenitor cells. NaBt also endows the neuronal cells with increased mitochondrial spare capacity to confer resistance to oxidative stress associated with neuronal differentiation. We demonstrate that mitochondrial biogenesis is under HDAC-mediated epigenetic regulation, the timing of which is consistent with its integrative role during neuronal differentiation. Thus, our findings add a new facet to our mechanistic understanding of how pan-HDACis induce differentiation of neuronal progenitor cells. Our results reveal the concept that epigenetic modulation of the mitochondrial pool prior to neurotrophic signaling dictates the efficiency of initiation of neuronal differentiation during the transition from progenitor to differentiating neuronal cells. The histone acetyltransferase CREB-binding protein plays a key role in regulating the mitochondrial biomass. By ChIP-seq analysis, we show that NaBt confers an H3K27ac epigenetic signature in several interconnected nodes of nuclear genes vital for neuronal differentiation and mitochondrial reprogramming. Collectively, our study reports a novel developmental epigenetic layer that couples mitochondrial biogenesis to neuronal differentiation.</p>',
	'date' => '2018-03-02',
	'pmid' => 'http://www.pubmed.gov/29500414',
	'doi' => '10.1038/s41419-018-0396-1',
	'modified' => '2019-02-15 21:21:45',
	'created' => '2019-02-14 15:01:22',
	'ProductsPublication' => array(
		'id' => '3104',
		'product_id' => '2599',
		'publication_id' => '3463'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/29500414" target="_blank"><i class="fa fa-external-link"></i></a>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Contact Information

First name

Last name

Company

Phone number

City

Country

State

Email verification^*

Comment