Jarid1c Antibody

Catalog Number

Format

Price

C15310143
(CS-143-100)

100 µl

$270.00
Bulk order

Alternative names: KDM5C, MRXJ, MRXSJ, SMCX, XE169

Polyclonal antibody raised in rabbit against mouse Jarid1c (jumonji, AT rich interactive domain 1C), using 3 KLH-conjugated synthetic peptides containing sequences from the inner part of the protein.

Lot	A474-001
Concentration	not determined
Species reactivity	Human, mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:500	Fig 1
Western Blotting	1:1,000	Fig 2

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Jarid1c (Cat. No. CS-143-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:7,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1c
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against Jarid1c (Cat. No. CS-143-100, lane 2) and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.

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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1c</strong><br />Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against Jarid1c (Cat. No. CS-143-100, lane 2) and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1055
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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<div class="small-6 columns">
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1c</strong><br />Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against Jarid1c (Cat. No. CS-143-100, lane 2) and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.</small></p>
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<td>Fig 1</td>
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<td>Fig 2</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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include - APP/View/Products/view.ctp, line 755
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1c</strong><br />Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against Jarid1c (Cat. No. CS-143-100, lane 2) and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.</small></p>
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		'name' => 'Jarid1c polyclonal antibody',
		'description' => 'Jarid1c (UniProtKB/Swiss-Prot entry P41229) is a histone demethylase which specifically demethylates lysine 4 of histone H3, thereby playing a central role in the regulation of transcription and chromatin remodelling. Jarid1c demethylates the trimethylated and dimethylated lysine 4, but not the monomethylated form. Further, Jarid1c participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Mutations in the Jarid1c gene have been associated with X-linked mental retardation.',
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		'lot' => 'A474-001',
		'concentration' => 'not determined',
		'reactivity' => 'Human, mouse',
		'type' => 'Polyclonal',
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		'classification' => 'Pioneer',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
</tbody>
</table>',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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Datasheet Jarid1c CS-143-100 DATASHEET Datasheet description	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

Jarid1c Antibody

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