DNA Methylation
Bisulfite conversion
Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
How it works
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
Figure 1: Overview of bisulfite conversion of DNA
Recommended products for Bisulfite conversion
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02030030 |
Premium Bisulfite kit Make your Bisulfite conversion now in only 60 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion... |
50 rxns | $245.00 | |
| C02030036 |
Premium RRBS kit V2 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the... |
24 rxns | $1,590.00 | |
| C02030037 |
Premium RRBS kit V2 x96 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s... |
96 rxns | $3,745.00 | |
| C02030031 |
Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
40 rxns | $245.00 | |
| C02030035 |
Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S... |
8 rxns | $125.00 | |
| C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets th... |
48 rxns | $285.00 | |
| C02030042 |
Premium Methyl UDI-UMI Adapters Module - 96 rxns Premium Methyl UDI-UMI Adapters Module - 96 rxns includes 96 methylated full-length adapters with unique dual indexes (UDI) and un... |
96 rxns | $950.00 | |
| C02030040 |
Premium Methyl UDI-UMI Adapters Module - Set A Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mo... |
24 rxns | $250.00 | |
| C02030041 |
Premium Methyl UDI-UMI Adapters Module - Set B Premium Methyl UDI-UMI Adapters Module - Set B includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mo... |
24 rxns | $250.00 |