Step by step workflow |
Optimal solution from Diagenode |
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1. Crosslink to bind proteins to DNA  |
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2. Shear DNA  |
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3. Immunoprecipitate with specific antibody  |
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4. Reverse crosslinks and purify  |
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5. ChIP-qPCR or ChIP-seq library preparation |
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Chromatin studies
Chromatin structure plays a key role in regulating gene expression by allowing DNA accessibility to transcriptional machinery and transcription factors. The packaging of DNA into nucleosomes forms a closed structure that is not highly accessible to transcriptional elements whereas the open nucleosome structure allows DNA to be accessible. Diagenode offers a number of solutions to help you analyze chromatin and the role of transcriptional machinery including ChIP kits, ChIPmentation kits, antibodies, pA-Tn5 and ATAC-seq kits.
Chromatin immunoprecipitation (ChIP) determines the location of DNA binding sites on the genome for a protein of interest, giving insights into gene expression regulation. ChIP involves the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a protein or protein modification are used to determine the relative abundance of that antigen at a specific locus or loci. ChIP can be used to compare enrichment of proteins, map protein modifications, or quantify a protein modification during a time course.
The ChIP workflow
Products for chromatin study
Crosslinking
Efficient solution for protein-protein fixation. Read more
Chromatin shearing
Perfectly sheared chromatin is critical for ChIP success.
Read more about solutions for successful chromatin preparation.
Read more about Bioruptor sonication.
Chromatin immunoprecipitation
Immunoprecipitation solutions for histone and transcription factor ChIP-qPCR and ChIP-seq for low inputs, plants, and animals including automated solutions. Read more
ChIPmentation
ChIPmentation, an exclusive technology, is an end-to-end ChIP-seq solution for low and difficult inputs. Read more
ChIP and ChIP-seq antibodies
ChIP-grade antibodies are essential for success. Learn more
Primer pairs
Highly specific primer pairs for the amplification of the specific genomic regions. Read more
CUT&Tag solutions
An alternative to ChIP-seq that combines antibody-targeted controlled cleavage by a protein A-Tn5 fusion with NGS to identify the binding sites of DNA-associated proteins. Read more
DNA purification
Read more about solutions for DNA purification
Library preparation for ChIP-seq
Optimized solutions for the library preparation from low DNA input. Read more
ChIP and ChIP-seq automation
Reproducibility, optimization simplicity, no variability. Learn more
We offer complete ChIP kits or individual kit components from antibodies, buffers, beads, chromatin shearing, and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.
Chromatin resources
Posters
- Understanding our antibody QC
- High quality ChIP antibodies
- ChIP with only 10,000 cells
- High resolution ChIP-seq using automation
- ChIP-seq bioinformatics
Application notes
- Simple semi-automaton for easy and inexpensive ChIP
- Performing ChIP-seq on human tumor tissue
- Plant ChIP-seq – a successful method
- Best workflow practices for low input ChIP
- Optimize the selection of guide RNA by ChIP to keep CRISPR on-target
Publications related to ChIP
- Corticosteroid receptors adopt distinct cyclical transcriptional signatures
- Pro-inflammatory cytokine and high doses of ionizing radiation have similar effects on the expression of NF-kappaB-dependent genes
- Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile
Brochures
Products
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C01080002 |
ATAC-seq kit Download the Manual ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by next generation sequencing, is a key technology for geno... |
24 rxns | $1,745.00 |
Chromatin shearing
Chromatin immunoprecipitation
ChIPmentation™
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C01011011 |
µChIPmentation Kit for Histones Download the Manual The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to ... |
24 rxns | $2,400.00 | |
| C01011032 |
24 SI for Tagmented libraries The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries ... |
24 SI | $190.00 | |
| C01011000 |
Auto ChIPmentation Kit for Histones - Replaced by C01011009 Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ... |
24 rxns | ||
| C01011010 |
ChIPmentation Kit for Histones - Replaced by C01011009 Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ... |
24 rxns | ||
| C01011030 |
TAG Kit for ChIPmentation Contact us Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fra... |
24 rxns | $1,050.00 |
