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Bioruptor^® Plus sonication device		Get a quote You are about to request a quote for Bioruptor^® Plus sonication device. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
0.1 ml Bioruptor^® Pico Microtubes	$250.00
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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 92 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 92] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 92 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118	(Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 98] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 98 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 99] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 99 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118	(Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 105] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 105 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 113] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 113 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 115] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 115 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 116] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 116 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = null CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 array_keys - [internal], line ?? include - APP/View/Elements/Forms/addtocart.ctp, line 5 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 116 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 将添加至我的购物车。 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 43 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 116 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 44 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 116 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' $options = array( 'class' => 'alert small button expand', 'onclick' => '$(this).addToCart('', '', '', $('#CartQuantity').val());', 'div' => false, 'name' => 'checkout', 'id' => 'checkout', 'value' => 'checkout' ) CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 54 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 116 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' $options = array( 'class' => 'alert small button expand', 'onclick' => '$(this).addToCart('', '', '', $('#CartQuantity').val());', 'div' => false, 'name' => 'checkout', 'id' => 'checkout', 'value' => 'checkout' ) CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 55 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 116 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 × (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 119] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells', (int) 3 => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array(), (int) 3 => array( 'Product' => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)', 'description' => '<p style="text-align: justify;">Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the <a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a> (< 0.1% SDS), the<span> </span><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for Transcription Factors</a> (0.2% SDS), the <a href="../p/highcell-chip-kit-protein-a-x16-16-rxns">HighCell# ChIP kit</a> (0.5% SDS) and the<span> </span><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span> (1% SDS)</span>, which allows you to first optimize your chromatin shearing before performing ChIP using our kits.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '310', 'price_USD' => '355', 'price_GBP' => '285', 'price_JPY' => '50795', 'price_CNY' => '', 'price_AUD' => '888', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2025-04-03 11:54:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 119 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? 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Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)	$355.00

Bioruptor^® Plus sonication device		Get a quote You are about to request a quote for Bioruptor^® Plus sonication device. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
0.1 ml Bioruptor^® Pico Microtubes	$250.00
ChIP Cross-link Gold	$165.00
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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 188 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 188] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 188 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118	(Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 195] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 195 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 196] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 196 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118	(Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 202] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 202 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 210] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 210 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? 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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 212 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 213] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 213 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = null CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 array_keys - [internal], line ?? include - APP/View/Elements/Forms/addtocart.ctp, line 5 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 213 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 将添加至我的购物车。 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 43 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 213 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 44 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 213 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' $options = array( 'class' => 'alert small button expand', 'onclick' => '$(this).addToCart('', '', '', $('#CartQuantity').val());', 'div' => false, 'name' => 'checkout', 'id' => 'checkout', 'value' => 'checkout' ) CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 54 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 213 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' $options = array( 'class' => 'alert small button expand', 'onclick' => '$(this).addToCart('', '', '', $('#CartQuantity').val());', 'div' => false, 'name' => 'checkout', 'id' => 'checkout', 'value' => 'checkout' ) CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 55 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 213 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 × (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 216] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '0-1-ml-bioruptor-microtubes-120-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '175', 'price_USD' => '250', 'price_GBP' => '150', 'price_JPY' => '28675', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 216 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118

ANTIBODIES

H3K4me3 Antibody - ChIP-seq Grade	$485.00
H3K27ac Antibody - ChIP-seq Grade	$485.00
H3K27me3 Antibody - ChIP-seq Grade	$485.00
Check out the complete histone family.

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 380]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
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				[maximum depth reached]
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		'Image' => array(
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		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 380
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 380]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 5 => array(
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			(int) 6 => array(
				[maximum depth reached]
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		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
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			(int) 3 => array(
				[maximum depth reached]
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			(int) 4 => array(
				[maximum depth reached]
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				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 380
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 387]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 387
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 388]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
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			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
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		'Image' => array(
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		'Promotion' => array(),
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			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
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			(int) 5 => array(
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			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 388
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 394]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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		'Promotion' => array(),
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			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
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	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 394
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 402]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 402
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 404]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 404
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 405]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
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				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 408]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 408
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 380]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 380
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 380]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 380
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 387]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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		),
		'Testimonial' => array(),
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		'SafetySheet' => array(
			(int) 0 => array(
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			(int) 1 => array(
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		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 387
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 388]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
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				[maximum depth reached]
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		'Image' => array(
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		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 388
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 394]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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				[maximum depth reached]
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			(int) 5 => array(
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			(int) 6 => array(
				[maximum depth reached]
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		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
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			(int) 3 => array(
				[maximum depth reached]
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			(int) 4 => array(
				[maximum depth reached]
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			(int) 6 => array(
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				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 394
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 402]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 402
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 404]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
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			(int) 3 => array(
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		'Image' => array(
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		'Promotion' => array(),
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			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
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			(int) 4 => array(
				[maximum depth reached]
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			(int) 5 => array(
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			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 404
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 405]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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				[maximum depth reached]
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		),
		'Category' => array(
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				[maximum depth reached]
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			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
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		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
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				[maximum depth reached]
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				[maximum depth reached]
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			(int) 5 => array(
				[maximum depth reached]
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			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 405
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 408]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
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			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
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			'format' => '100 µl',
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			'sf_code' => 'C15310197-D001-001161',
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			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
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			'price_JPY' => '63900',
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			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
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			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
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			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'modified' => '2022-02-24 14:04:47',
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AML1-ETO Antibody - ChIP-seq Grade

$390.00

Check out the complete transcription factor family.

ChIP KITS

iDeal ChIP-qPCR Kit	$560.00
iDeal ChIP-seq kit for Histones	$1,130.00
True MicroChIP Kit	$680.00

iDeal ChIP-qPCR Kit	$560.00
iDeal ChIP-seq kit for Transcription Factors	$1,130.00

LIBRARY PREPARATION KITS

MicroPlex Library Preparation Kit v2 (12 indexes) $1,250.00

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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 645]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 645
View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 646]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 646
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 652]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 652
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 660]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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$title_for_layout = 'Shopping-list'
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
			'label2' => '',
			'info2' => '',
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			'format' => '12 rxns',
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			'type' => 'FRE',
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			'meta_title' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'meta_keywords' => '',
			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
			'created' => '2015-06-29 14:08:20',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 660
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 662]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 662
View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 663]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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$title_for_layout = 'Shopping-list'
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 663
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View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
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将添加至我的购物车。

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include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 663
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 663
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 663
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 663
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 666]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
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)
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '12 rxns',
			'catalog_number' => 'C05010012',
			'old_catalog_number' => 'C05010010',
			'sf_code' => 'C05010012-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '955',
			'price_USD' => '1250',
			'price_GBP' => '855',
			'price_JPY' => '156475',
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			'featured' => false,
			'no_promo' => false,
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			'last_datasheet_update' => '0000-00-00',
			'slug' => 'microplex-library-preparation-kit-v2-x12-12-indices-12-rxns',
			'meta_title' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'meta_keywords' => '',
			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
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ReflectionMethod::invokeArgs() - [internal], line ??
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MicroPlex Library Preparation Kit v2 (12 indexes) $1,250.00

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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 710
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 710]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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$page = 'portal'
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
			'label2' => '',
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			'format' => '12 rxns',
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			'meta_keywords' => '',
			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 710
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 724]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
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<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			(int) 22 => array(
				[maximum depth reached]
			),
			(int) 23 => array(
				[maximum depth reached]
			),
			(int) 24 => array(
				[maximum depth reached]
			),
			(int) 25 => array(
				[maximum depth reached]
			),
			(int) 26 => array(
				[maximum depth reached]
			),
			(int) 27 => array(
				[maximum depth reached]
			),
			(int) 28 => array(
				[maximum depth reached]
			),
			(int) 29 => array(
				[maximum depth reached]
			),
			(int) 30 => array(
				[maximum depth reached]
			),
			(int) 31 => array(
				[maximum depth reached]
			),
			(int) 32 => array(
				[maximum depth reached]
			),
			(int) 33 => array(
				[maximum depth reached]
			),
			(int) 34 => array(
				[maximum depth reached]
			),
			(int) 35 => array(
				[maximum depth reached]
			),
			(int) 36 => array(
				[maximum depth reached]
			),
			(int) 37 => array(
				[maximum depth reached]
			),
			(int) 38 => array(
				[maximum depth reached]
			),
			(int) 39 => array(
				[maximum depth reached]
			),
			(int) 40 => array(
				[maximum depth reached]
			),
			(int) 41 => array(
				[maximum depth reached]
			),
			(int) 42 => array(
				[maximum depth reached]
			),
			(int) 43 => array(
				[maximum depth reached]
			),
			(int) 44 => array(
				[maximum depth reached]
			),
			(int) 45 => array(
				[maximum depth reached]
			),
			(int) 46 => array(
				[maximum depth reached]
			),
			(int) 47 => array(
				[maximum depth reached]
			),
			(int) 48 => array(
				[maximum depth reached]
			),
			(int) 49 => array(
				[maximum depth reached]
			),
			(int) 50 => array(
				[maximum depth reached]
			),
			(int) 51 => array(
				[maximum depth reached]
			),
			(int) 52 => array(
				[maximum depth reached]
			),
			(int) 53 => array(
				[maximum depth reached]
			),
			(int) 54 => array(
				[maximum depth reached]
			),
			(int) 55 => array(
				[maximum depth reached]
			),
			(int) 56 => array(
				[maximum depth reached]
			),
			(int) 57 => array(
				[maximum depth reached]
			),
			(int) 58 => array(
				[maximum depth reached]
			),
			(int) 59 => array(
				[maximum depth reached]
			),
			(int) 60 => array(
				[maximum depth reached]
			),
			(int) 61 => array(
				[maximum depth reached]
			),
			(int) 62 => array(
				[maximum depth reached]
			),
			(int) 63 => array(
				[maximum depth reached]
			),
			(int) 64 => array(
				[maximum depth reached]
			),
			(int) 65 => array(
				[maximum depth reached]
			),
			(int) 66 => array(
				[maximum depth reached]
			),
			(int) 67 => array(
				[maximum depth reached]
			),
			(int) 68 => array(
				[maximum depth reached]
			),
			(int) 69 => array(
				[maximum depth reached]
			),
			(int) 70 => array(
				[maximum depth reached]
			),
			(int) 71 => array(
				[maximum depth reached]
			),
			(int) 72 => array(
				[maximum depth reached]
			),
			(int) 73 => array(
				[maximum depth reached]
			),
			(int) 74 => array(
				[maximum depth reached]
			),
			(int) 75 => array(
				[maximum depth reached]
			),
			(int) 76 => array(
				[maximum depth reached]
			),
			(int) 77 => array(
				[maximum depth reached]
			),
			(int) 78 => array(
				[maximum depth reached]
			),
			(int) 79 => array(
				[maximum depth reached]
			),
			(int) 80 => array(
				[maximum depth reached]
			),
			(int) 81 => array(
				[maximum depth reached]
			),
			(int) 82 => array(
				[maximum depth reached]
			),
			(int) 83 => array(
				[maximum depth reached]
			),
			(int) 84 => array(
				[maximum depth reached]
			),
			(int) 85 => array(
				[maximum depth reached]
			),
			(int) 86 => array(
				[maximum depth reached]
			),
			(int) 87 => array(
				[maximum depth reached]
			),
			(int) 88 => array(
				[maximum depth reached]
			),
			(int) 89 => array(
				[maximum depth reached]
			),
			(int) 90 => array(
				[maximum depth reached]
			),
			(int) 91 => array(
				[maximum depth reached]
			),
			(int) 92 => array(
				[maximum depth reached]
			),
			(int) 93 => array(
				[maximum depth reached]
			),
			(int) 94 => array(
				[maximum depth reached]
			),
			(int) 95 => array(
				[maximum depth reached]
			),
			(int) 96 => array(
				[maximum depth reached]
			),
			(int) 97 => array(
				[maximum depth reached]
			),
			(int) 98 => array(
				[maximum depth reached]
			),
			(int) 99 => array(
				[maximum depth reached]
			),
			(int) 100 => array(
				[maximum depth reached]
			),
			(int) 101 => array(
				[maximum depth reached]
			),
			(int) 102 => array(
				[maximum depth reached]
			),
			(int) 103 => array(
				[maximum depth reached]
			),
			(int) 104 => array(
				[maximum depth reached]
			),
			(int) 105 => array(
				[maximum depth reached]
			),
			(int) 106 => array(
				[maximum depth reached]
			),
			(int) 107 => array(
				[maximum depth reached]
			),
			(int) 108 => array(
				[maximum depth reached]
			),
			(int) 109 => array(
				[maximum depth reached]
			),
			(int) 110 => array(
				[maximum depth reached]
			),
			(int) 111 => array(
				[maximum depth reached]
			),
			(int) 112 => array(
				[maximum depth reached]
			),
			(int) 113 => array(
				[maximum depth reached]
			),
			(int) 114 => array(
				[maximum depth reached]
			),
			(int) 115 => array(
				[maximum depth reached]
			),
			(int) 116 => array(
				[maximum depth reached]
			),
			(int) 117 => array(
				[maximum depth reached]
			),
			(int) 118 => array(
				[maximum depth reached]
			),
			(int) 119 => array(
				[maximum depth reached]
			),
			(int) 120 => array(
				[maximum depth reached]
			),
			(int) 121 => array(
				[maximum depth reached]
			),
			(int) 122 => array(
				[maximum depth reached]
			),
			(int) 123 => array(
				[maximum depth reached]
			),
			(int) 124 => array(
				[maximum depth reached]
			),
			(int) 125 => array(
				[maximum depth reached]
			),
			(int) 126 => array(
				[maximum depth reached]
			),
			(int) 127 => array(
				[maximum depth reached]
			),
			(int) 128 => array(
				[maximum depth reached]
			),
			(int) 129 => array(
				[maximum depth reached]
			),
			(int) 130 => array(
				[maximum depth reached]
			),
			(int) 131 => array(
				[maximum depth reached]
			),
			(int) 132 => array(
				[maximum depth reached]
			),
			(int) 133 => array(
				[maximum depth reached]
			),
			(int) 134 => array(
				[maximum depth reached]
			),
			(int) 135 => array(
				[maximum depth reached]
			),
			(int) 136 => array(
				[maximum depth reached]
			),
			(int) 137 => array(
				[maximum depth reached]
			),
			(int) 138 => array(
				[maximum depth reached]
			),
			(int) 139 => array(
				[maximum depth reached]
			),
			(int) 140 => array(
				[maximum depth reached]
			),
			(int) 141 => array(
				[maximum depth reached]
			),
			(int) 142 => array(
				[maximum depth reached]
			),
			(int) 143 => array(
				[maximum depth reached]
			),
			(int) 144 => array(
				[maximum depth reached]
			),
			(int) 145 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			)
		),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	(int) 1 => array()
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 735
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 735
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 735
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 735
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 735
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 735
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 738]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'microplex-library-preparation-kit-v2-x12-12-indices-12-rxns',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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Automated solutions for use with IP-Star

Your ChIP shopping cart for automated ChIP for use with the IP-Star

AUTOMATED HISTONE ChIP

AUTOMATED TRANSCRIPTION FACTOR ChIP

SHEARING

Bioruptor^® Plus sonication device

1.5 ml Bioruptor^® Pico Microtubes with Caps

$165.00

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			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
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		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array()
	),
	(int) 2 => array()
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 806
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 806]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'bioruptor-plus-sonication-device',
	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
	(int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells'
)
$products = array(
	(int) 0 => array(
		'Product' => array(
			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
			'format' => '1 unit',
			'catalog_number' => 'B01020014',
			'old_catalog_number' => 'UCD-300 TM',
			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
			'price_EUR' => '23000',
			'price_USD' => '30400',
			'price_GBP' => '19900',
			'price_JPY' => '3768550',
			'price_CNY' => '',
			'price_AUD' => '76000',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => true,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bioruptor-plus-sonication-device',
			'meta_title' => 'Bioruptor® Plus sonication device',
			'meta_keywords' => '',
			'meta_description' => 'Bioruptor® Plus sonication device',
			'modified' => '2025-06-25 10:33:17',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
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			'modified' => null,
			'created' => null,
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		'Product' => array(
			'id' => '2630',
			'antibody_id' => null,
			'name' => '1.5 ml Bioruptor<sup>&reg;</sup> Pico Microtubes with Caps',
			'description' => '<p><span>The 1.5 ml Bioruptor<sup>&reg;</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>&reg;</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument.&nbsp;Recommended sample volume: 100 &mu;l - 300 &mu;l.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
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			'catalog_number' => 'C30010016',
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			'sf_code' => 'C30010016-5700',
			'type' => 'ACC',
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			'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps',
			'meta_keywords' => '',
			'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps',
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 806
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 813]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'bioruptor-plus-sonication-device',
	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
	(int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells'
)
$products = array(
	(int) 0 => array(
		'Product' => array(
			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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			'catalog_number' => 'B01020014',
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			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 813
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 814]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
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	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
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			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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			'old_catalog_number' => 'UCD-300 TM',
			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
			'price_EUR' => '23000',
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			'meta_description' => 'Bioruptor® Plus sonication device',
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			'description' => '<p><span>The 1.5 ml Bioruptor<sup>&reg;</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>&reg;</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument.&nbsp;Recommended sample volume: 100 &mu;l - 300 &mu;l.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
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			'format' => '300 tubes',
			'catalog_number' => 'C30010016',
			'old_catalog_number' => '',
			'sf_code' => 'C30010016-5700',
			'type' => 'ACC',
			'search_order' => '01-Accessory',
			'price_EUR' => '115',
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			'meta_keywords' => '',
			'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps',
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			'created' => '2015-06-29 14:08:20',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 814
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 820]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
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	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
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			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
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<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
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			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
			'format' => '1 unit',
			'catalog_number' => 'B01020014',
			'old_catalog_number' => 'UCD-300 TM',
			'sf_code' => 'B01020001-',
			'type' => 'ACC',
			'search_order' => '00-Machine',
			'price_EUR' => '23000',
			'price_USD' => '30400',
			'price_GBP' => '19900',
			'price_JPY' => '3768550',
			'price_CNY' => '',
			'price_AUD' => '76000',
			'country' => 'ALL',
			'except_countries' => 'Japan',
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			'slug' => 'bioruptor-plus-sonication-device',
			'meta_title' => 'Bioruptor® Plus sonication device',
			'meta_keywords' => '',
			'meta_description' => 'Bioruptor® Plus sonication device',
			'modified' => '2025-06-25 10:33:17',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
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		'Product' => array(
			'id' => '2630',
			'antibody_id' => null,
			'name' => '1.5 ml Bioruptor<sup>&reg;</sup> Pico Microtubes with Caps',
			'description' => '<p><span>The 1.5 ml Bioruptor<sup>&reg;</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>&reg;</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument.&nbsp;Recommended sample volume: 100 &mu;l - 300 &mu;l.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label1' => '',
			'info1' => '<p></p>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '300 tubes',
			'catalog_number' => 'C30010016',
			'old_catalog_number' => '',
			'sf_code' => 'C30010016-5700',
			'type' => 'ACC',
			'search_order' => '01-Accessory',
			'price_EUR' => '115',
			'price_USD' => '165',
			'price_GBP' => '110',
			'price_JPY' => '18845',
			'price_CNY' => '',
			'price_AUD' => '412',
			'country' => 'ALL',
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			'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
			'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps',
			'meta_keywords' => '',
			'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps',
			'modified' => '2022-07-19 05:04:02',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 828
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 830]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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$language = 'cn'
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$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'bioruptor-plus-sonication-device',
	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
	(int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells'
)
$products = array(
	(int) 0 => array(
		'Product' => array(
			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
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			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 831]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'bioruptor-plus-sonication-device',
	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
	(int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells'
)
$products = array(
	(int) 0 => array(
		'Product' => array(
			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
			'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps',
			'meta_keywords' => '',
			'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps',
			'modified' => '2022-07-19 05:04:02',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
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			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
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			(int) 3 => array(
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		'Publication' => array(
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		'Testimonial' => array(),
		'Area' => array(),
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 831
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
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	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 831
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 831
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
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	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 831
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 831
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
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	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
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	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 831
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 834]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
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	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'bioruptor-plus-sonication-device',
	(int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes',
	(int) 2 => 'chromatin-shearing-optimization-kit-low-sds-100-million-cells'
)
$products = array(
	(int) 0 => array(
		'Product' => array(
			'id' => '1784',
			'antibody_id' => null,
			'name' => 'Bioruptor<sup>&reg;</sup> Plus sonication device',
			'description' => '<p><span>The Bioruptor&reg; uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
			'label1' => 'Which Bioruptor® Plus configuration is right for you?',
			'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples)&nbsp;tube holder <span>&amp; temperature controlled system (water cooler + single cycle valve)&nbsp;</span><em>for recommended sample volume 100&nbsp;&micro;l - 300&nbsp;&micro;l</em></p>
<p><strong>B01020002</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 1.5&nbsp;ml (6 samples) and 15 ml&nbsp;tube holders <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 100&nbsp;&micro;l - 2 ml</em></p>
<p><strong>B01020003</strong> -&nbsp;Bioruptor Plus<sup>&reg;</sup>&nbsp;device with 0.5 ml (12 samples)&nbsp;tube holder <span>&amp; temperature controlled system&nbsp;(water cooler + single cycle valve)</span>&nbsp;<em>for recommended sample volume 50&nbsp;&micro;l - 100&nbsp;&micro;l&nbsp;</em></p>',
			'label2' => 'Available chromatin shearing kits',
			'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">&lt; 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
			'label3' => '',
			'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor&reg;?</a></p>',
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Bioruptor^® Plus sonication device		Get a quote You are about to request a quote for Bioruptor^® Plus sonication device. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
1.5 ml Bioruptor^® Pico Microtubes with Caps	$165.00
ChIP Cross-link Gold	$165.00
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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 900 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 900] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 900 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? 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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2630', 'antibody_id' => null, 'name' => '1.5 ml Bioruptor<sup>®</sup> Pico Microtubes with Caps', 'description' => '<p><span>The 1.5 ml Bioruptor<sup>®</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 907 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 908] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2630', 'antibody_id' => null, 'name' => '1.5 ml Bioruptor<sup>®</sup> Pico Microtubes with Caps', 'description' => '<p><span>The 1.5 ml Bioruptor<sup>®</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 908 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118	(Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 914] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2630', 'antibody_id' => null, 'name' => '1.5 ml Bioruptor<sup>®</sup> Pico Microtubes with Caps', 'description' => '<p><span>The 1.5 ml Bioruptor<sup>®</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. 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Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 914 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 922] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2630', 'antibody_id' => null, 'name' => '1.5 ml Bioruptor<sup>®</sup> Pico Microtubes with Caps', 'description' => '<p><span>The 1.5 ml Bioruptor<sup>®</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. 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Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 924] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 924 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? 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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 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reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ), (int) 1 => array( 'Product' => array( 'id' => '2630', 'antibody_id' => null, 'name' => '1.5 ml Bioruptor<sup>®</sup> Pico Microtubes with Caps', 'description' => '<p><span>The 1.5 ml Bioruptor<sup>®</sup> Microtubes for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 925 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = null CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 array_keys - [internal], line ?? include - APP/View/Elements/Forms/addtocart.ctp, line 5 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 925 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 将添加至我的购物车。 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 43 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 925 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 44 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 925 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' $options = array( 'class' => 'alert small button expand', 'onclick' => '$(this).addToCart('', '', '', $('#CartQuantity').val());', 'div' => false, 'name' => 'checkout', 'id' => 'checkout', 'value' => 'checkout' ) CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 54 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 925 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list', 'product' => null ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $product = array( 'name' => null, 'id' => null ) $id = null $quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>' $options = array( 'class' => 'alert small button expand', 'onclick' => '$(this).addToCart('', '', '', $('#CartQuantity').val());', 'div' => false, 'name' => 'checkout', 'id' => 'checkout', 'value' => 'checkout' ) CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Elements/Forms/addtocart.ctp, line 55 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Pages/portal/shopping-list.ctp, line 925 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118 × (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 928] Code Context `$errorType = self::getErrorType($code);` `self::logToDatabase( $errorType, $description, $file, $line);` `return parent::handleError( $errorType, $description, $file, $line, $context);` $viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'cn', 'page' => 'portal', 'subpage' => 'shopping-list', 'title_for_layout' => 'Shopping-list' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'cn' $page = 'portal' $subpage = 'shopping-list' $title_for_layout = 'Shopping-list' $url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis' $slugs = array( (int) 0 => 'bioruptor-plus-sonication-device', (int) 1 => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', (int) 2 => 'chip-cross-link-gold-600-ul', (int) 3 => 'chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns' ) $products = array( (int) 0 => array( 'Product' => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => '', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020014', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '23000', 'price_USD' => '30400', 'price_GBP' => '19900', 'price_JPY' => '3768550', 'price_CNY' => '', 'price_AUD' => '76000', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2025-06-25 10:33:17', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), 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These tubes have been thoroughly validated for use on the Bioruptor<sup>®</sup> Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '115', 'price_USD' => '165', 'price_GBP' => '110', 'price_JPY' => '18845', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), (int) 2 => array( 'Product' => array( 'id' => '1866', 'antibody_id' => null, 'name' => 'ChIP Cross-link Gold', 'description' => '<p style="text-align: justify;"><span>Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective </span><span>in cross-linking</span><span> proteins that are not directly bound to the DNA.</span><span> </span><span>For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. F</span><span>or such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is</span><span> used in combination with formaldehyde is an excellent choice for such higher order protein interactions. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 µl', 'catalog_number' => 'C01019027', 'old_catalog_number' => '', 'sf_code' => 'C01019027-50620', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '195', 'price_USD' => '165', 'price_GBP' => '175', 'price_JPY' => '31950', 'price_CNY' => '', 'price_AUD' => '412', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chip-cross-link-gold-600-ul', 'meta_title' => 'Chromatin immunoprecipitation(ChIP) Cross-linking Gold \| Diagenode', 'meta_keywords' => 'ChIP Cross-link Gold,Chromatin immunoprecipitation(ChIP) Cross-linking Gold,DNA-protein,reagent,formaldehyde', 'meta_description' => 'Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.For higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChI', 'modified' => '2020-05-27 13:37:24', 'created' => '2015-06-29 14:08:20', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '***', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), (int) 3 => array() ) $product = array() CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9 include - APP/View/Pages/portal/shopping-list.ctp, line 928 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 PagesController::display() - APP/Controller/PagesController.php, line 150 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118

ANTIBODIES

H3K4me3 Antibody - ChIP-seq Grade	$485.00
H3K27ac Antibody - ChIP-seq Grade	$485.00
H3K27me3 Antibody - ChIP-seq Grade	$485.00
Check out the complete histone family.

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1091]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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$dataForView = array(
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		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1091
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1091]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
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	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
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$products = array(
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			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1091
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1098]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1098
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1099]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1099
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1105]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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				[maximum depth reached]
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		'Publication' => array(
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				[maximum depth reached]
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			(int) 6 => array(
				[maximum depth reached]
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		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
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				[maximum depth reached]
			),
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				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1105
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1113]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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				[maximum depth reached]
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		'Testimonial' => array(),
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		'SafetySheet' => array(
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	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1113
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1115]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
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				[maximum depth reached]
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				[maximum depth reached]
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				[maximum depth reached]
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			(int) 3 => array(
				[maximum depth reached]
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		),
		'Document' => array(
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				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
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		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
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				[maximum depth reached]
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			(int) 3 => array(
				[maximum depth reached]
			),
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				[maximum depth reached]
			),
			(int) 5 => array(
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			),
			(int) 6 => array(
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			)
		),
		'Testimonial' => array(),
		'Area' => array(),
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				[maximum depth reached]
			),
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				[maximum depth reached]
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				[maximum depth reached]
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			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1115
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1116]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
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			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
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		'Document' => array(
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		'Protocol' => array(),
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				[maximum depth reached]
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			(int) 3 => array(
				[maximum depth reached]
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			(int) 4 => array(
				[maximum depth reached]
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			(int) 5 => array(
				[maximum depth reached]
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			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1119]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1119
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1091]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
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		'Testimonial' => array(),
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)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1091
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1091]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
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				[maximum depth reached]
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		'SafetySheet' => array(
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	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1091
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1098]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
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			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1098
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1099]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
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			(int) 1 => array(
				[maximum depth reached]
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				[maximum depth reached]
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			(int) 6 => array(
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		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
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			(int) 1 => array(
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				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1099
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1105]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1105
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1113]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1113
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1115]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
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		'Testimonial' => array(),
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		'SafetySheet' => array(
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	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1115
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1116]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
		),
		'Related' => array(),
		'Application' => array(
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				[maximum depth reached]
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		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
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		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
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				[maximum depth reached]
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				[maximum depth reached]
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				[maximum depth reached]
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			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1116
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1119]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'pol-ii-monoclonal-antibody-classic-50-mg-50-ml',
	(int) 1 => 'ctcf-polyclonal-antibody-classic-50-mg-20-ml',
	(int) 2 => 'aml1-eto-polyclonal-antibody-classic-100-ul'
)
$products = array(
	(int) 0 => array(),
	(int) 1 => array(),
	(int) 2 => array(
		'Product' => array(
			'id' => '2135',
			'antibody_id' => '65',
			'name' => 'AML1-ETO Antibody - ChIP-seq Grade',
			'description' => '<p><span>Alternative name: <strong>RUNX1-RUNX1T1 <br /></strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against the <strong>AML1-ETO</strong> fusion protein using a KLH-conjugated synthetic peptide.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIP.png" alt="AML1-ETO Antibody ChIP Grade" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against AML1-ETO</strong><br />ChIP assays were performed using Kasumi-1 cells, the Diagenode antibody against AML1-ETO (Cat. No. C15310197) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong>ChIP-seq signals on chromosome 3</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-A.png" alt="AML1-ETO Antibody ChIP-seq Grade" /><br /><small><strong>B.</strong>Genomic region on chromosome 3 surrounding the OGG1 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-B.png" alt="AML1-ETO Antibody for ChIP-seq" /><br /><small><strong>C. </strong>Genomic region on chromosome 9 surrounding the FUT7 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-C.png" alt="AML1-ETO Antibody for ChIP-seq assay" /><br /><small><strong>D. </strong>Genomic region on chromosome 12 surrounding the NFE2 gene</small><br /> <img src="https://www.diagenode.com/img/product/antibodies/C15310197_ChIPseq-D.png" alt="AML1-ETO Antibody validated in ChIP-seq" /></p>
<p></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AML1-ETO </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 6 ChIP&rsquo;s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197-elisa.png" alt="AML1-ETO Antibody ELISA Validation" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AML1-ETO (Cat. No. C15310197). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310197_WB.png" alt="AML1-ETO Antibody validated in  Western Blot" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against AML1-ETO</strong><br />Nuclear extracts of SKNO-1 cells (15 &mu;g) were analysed by Western blot using the Diagenode antibody against AML1-ETO (Cat. No. C15310197) diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5&rsquo;-region of AML1and the 3&rsquo;-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.</p>',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310197',
			'old_catalog_number' => '',
			'sf_code' => 'C15310197-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '390',
			'price_USD' => '390',
			'price_GBP' => '345',
			'price_JPY' => '63900',
			'price_CNY' => '',
			'price_AUD' => '975',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'aml1-eto-polyclonal-antibody-classic-100-ul',
			'meta_title' => 'AML1-ETO Antibody  - ChIP-seq Grade (C15310197) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'AML1-ETO Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: RUNX1-RUNX1T1 ',
			'modified' => '2022-02-24 14:07:39',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'zho'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '65',
			'name' => 'AML1-ETO polyclonal antibody',
			'description' => 'This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukaemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A706-002',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Classic',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 &mu;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 &mu;l per IP.</small></p>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2022-02-24 14:04:47',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '65 - AML1-ETO polyclonal antibody (A706-002 - not determined - Human - Whole antiserum - Rabbit)'
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AML1-ETO Antibody - ChIP-seq Grade

$390.00

Check out the complete transcription factor family.

ChIP KITS

Auto iDeal ChIP-seq kit for Histones

$2,340.00

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			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
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<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1190
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1190]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
			'label3' => 'Additional solutions compatible with Auto iDeal ChIP-seq Kit for Histones',
			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1190
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1197]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
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			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1197
View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1198]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
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<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
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			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1198
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1204]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
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<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1212]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'auto-ideal-chip-seq-kit-for-histones-x100-100-rxns',
	(int) 1 => 'auto-true-microchip-kit-16-rxns'
)
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			'id' => '2685',
			'antibody_id' => null,
			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
			'label3' => 'Additional solutions compatible with Auto iDeal ChIP-seq Kit for Histones',
			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1212
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1214]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
$slugs = array(
	(int) 0 => 'auto-ideal-chip-seq-kit-for-histones-x100-100-rxns',
	(int) 1 => 'auto-true-microchip-kit-16-rxns'
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			'id' => '2685',
			'antibody_id' => null,
			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
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			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1214
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1215]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
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$page = 'portal'
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			'id' => '2685',
			'antibody_id' => null,
			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
			'label3' => 'Additional solutions compatible with Auto iDeal ChIP-seq Kit for Histones',
			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
			'format' => '100 rxns',
			'catalog_number' => 'C01010171',
			'old_catalog_number' => '',
			'sf_code' => 'C01010171-',
			'type' => 'RFR',
			'search_order' => '',
			'price_EUR' => '2230',
			'price_USD' => '2340',
			'price_GBP' => '2040',
			'price_JPY' => '365385',
			'price_CNY' => '',
			'price_AUD' => '5850',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-ideal-chip-seq-kit-for-histones-x100-100-rxns',
			'meta_title' => 'Auto iDeal ChIP-seq kit for Histones | Diagenode',
			'meta_keywords' => 'Next-Generation Sequencing,iDeal ChIP-seq kit,Diagenode',
			'meta_description' => 'Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for GAIIx (Illumina®) and PGM™ (Ion Torrent™).',
			'modified' => '2021-01-19 16:26:17',
			'created' => '2015-09-09 10:35:24',
			'locale' => 'zho'
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$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1215
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
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	'page' => 'portal',
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	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
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$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1215
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
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	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1215
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
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	'title_for_layout' => 'Shopping-list',
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)
$carrousel_order = array(
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)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1215
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
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	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
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$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1215
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
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include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1215
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1218]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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			'name' => 'Auto iDeal ChIP-seq kit for Histones',
			'description' => '<p><span><strong>This product must be used with the <a href="https://www.diagenode.com/en/p/sx-8g-ip-star-compact-automated-system-1-unit">IP-Star Compact Automated System</a>.</strong></span></p>
<p><span>Don&rsquo;t risk wasting your precious sequencing samples. Diagenode&rsquo;s validated&nbsp;iDeal ChIP-seq kit&nbsp;has everything you need for a successful ChIP prior to Next-Generation Sequencing.The iDeal ChIP-seq kit is the only kit on the market validated for&nbsp;GAIIx (Illumina<sup>&reg;</sup>)&nbsp;and&nbsp;PGM&trade; (Ion Torrent&trade;). Our expertise in ChIP-seq tools allows reproducible and efficient results every time. Now, we also offer the&nbsp;</span>iDeal Library Preparation kit<span>&nbsp;to use in conjunction with the iDeal ChIP-seq kit.</span></p>',
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<li><strong>Validated:</strong> Only kit on the market validated for GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;)</li>
<li><strong>Proven:</strong> Our expertise with ChIP-seq tools enables reproducible and efficient results every time</li>
<li><strong>Complete:</strong> Kit includes control antibodies, control primer pairs and IPure DNA purification module</li>
</ul>
<p></p>
<p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent&trade; PGM&trade; (Life Technologies) and GAIIx (Illumina<sup>&reg;</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 &micro;g of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>&reg;</sup>) and PGM&trade; (Ion Torrent&trade;). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>&reg;</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>&reg;</sup> combined with the Bioruptor<sup>&reg;</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds &ldquo;ON&rdquo; / 30 seconds &ldquo;OFF&rdquo; at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4&deg;C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p>
<p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode&rsquo;s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p>',
			'label2' => 'Species, cell lines, tissues tested',
			'info2' => '<div id="info2" class="content active">
<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.</p>
<p><u>Cell lines:</u></p>
<p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p>
<p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p>
<p>Hamster: CHO</p>
<p><u>Tissues</u></p>
<p>Bee &ndash; brain</p>
<p>Daphnia &ndash; whole animal</p>
<p>Horse &ndash; brain, heart, lamina, liver, lung, skeletal muscles, ovary</p>
<p>Human &ndash; Erwing sarcoma tumor samples</p>
<p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&amp;body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>
</div>',
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			'info3' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p>&nbsp;The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal &nbsp;library preparation of ChIPed samples.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1218
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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ReflectionMethod::invokeArgs() - [internal], line ??
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Auto iDeal ChIP-qPCR Kit for Histones
Coming soon

Auto iDeal ChIP-seq Kit for Transcription Factors	$2,340.00
Auto iDeal ChIP-qPCR Kit for TFs Coming soon

LIBRARY PREPARATION KITS

MicroPlex Library Preparation Kit v2 (12 indexes) $1,250.00

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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1373]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1373
View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1375]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1375
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1376]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'meta_keywords' => '',
			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1376
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Warning): array_keys() expects parameter 1 to be array, null given [APP/View/Elements/Forms/addtocart.ctp, line 5]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = null
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1376
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1376
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1376
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
	'class' => 'alert small button expand',
	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1376
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
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	'onclick' => '$(this).addToCart('',
                    '',
                     '',
                       $('#CartQuantity').val());',
	'div' => false,
	'name' => 'checkout',
	'id' => 'checkout',
	'value' => 'checkout'
)
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1376
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1379]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Pages/portal/shopping-list.ctp'
$dataForView = array(
	'carrousel_order' => array(
		(int) 0 => 'Gene editing'
	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$url = 'https://dev.diagenode.com/applications/slug/slug:protein-analysis'
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	(int) 0 => 'microplex-library-preparation-kit-v2-x12-12-indices-12-rxns',
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'label3' => '',
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			'format' => '12 rxns',
			'catalog_number' => 'C05010012',
			'old_catalog_number' => 'C05010010',
			'sf_code' => 'C05010012-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '955',
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			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
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MicroPlex Library Preparation Kit v2 (12 indexes) $1,250.00

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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1423]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1430]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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include - APP/View/Pages/portal/shopping-list.ctp, line 1430
View::_evaluate() - CORE/Cake/View/View.php, line 971
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View::render() - CORE/Cake/View/View.php, line 473
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PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1431]Code Context         $errorType = self::getErrorType($code);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1431
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1437]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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			'id' => '1927',
			'antibody_id' => null,
			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'meta_keywords' => '',
			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1437
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1445]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
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<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1445
View::_evaluate() - CORE/Cake/View/View.php, line 971
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ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1447]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
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<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1448
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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$carrousel_order = array(
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
array_keys - [internal], line ??
include - APP/View/Elements/Forms/addtocart.ctp, line 5
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1448
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

将添加至我的购物车。

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 43]Code Context         $errorType = self::getErrorType($code);
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$carrousel_order = array(
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 43
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1448
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 44]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
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	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
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)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 44
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1448
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: catalog_number [APP/View/Elements/Forms/addtocart.ctp, line 54]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
$dataForView = array(
	'carrousel_order' => array(
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	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
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)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
$options = array(
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	'onclick' => '$(this).addToCart('',
                    '',
                     '',
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	'id' => 'checkout',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 54
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1448
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: price_USD [APP/View/Elements/Forms/addtocart.ctp, line 55]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
$viewFile = '/var/www/dev.diagenode.com/app/View/Elements/Forms/addtocart.ctp'
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	),
	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list',
	'product' => null
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
$product = array(
	'name' => null,
	'id' => null
)
$id = null
$quantity_input = '<input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/>'
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	'onclick' => '$(this).addToCart('',
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	'id' => 'checkout',
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CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Elements/Forms/addtocart.ctp, line 55
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Pages/portal/shopping-list.ctp, line 1448
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

 (Notice): Undefined index: Product [APP/View/Pages/portal/shopping-list.ctp, line 1451]Code Context         $errorType = self::getErrorType($code);
        self::logToDatabase( $errorType, $description, $file, $line);
        return parent::handleError( $errorType, $description, $file, $line, $context);
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$dataForView = array(
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	'language' => 'cn',
	'page' => 'portal',
	'subpage' => 'shopping-list',
	'title_for_layout' => 'Shopping-list'
)
$carrousel_order = array(
	(int) 0 => 'Gene editing'
)
$language = 'cn'
$page = 'portal'
$subpage = 'shopping-list'
$title_for_layout = 'Shopping-list'
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			'id' => '1927',
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			'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)',
			'description' => '<p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation&trade; kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the&nbsp;</span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results!&nbsp;</span></p>
<p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li>
<li><strong>Input: </strong>50 pg &ndash; 50 ng</li>
<li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li>
<li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li>
<li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li>
<li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>&reg;</sup> Automated Platform</a></strong></li>
</ul>
<h3>How it works</h3>
<center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center>
<p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina&reg; NGS platforms using a fast and simple 3-step protocol</small></p>
<ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;">
<li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a>
<div id="first" class="content">
<p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p>
<p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p>
<p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p>
<p><small>In the next step, stem-loop adaptors with blocked 5&rsquo; ends are ligated with high efficiency to the 5&rsquo; end of the genomic DNA, leaving a nick at the 3&rsquo; end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p>
<p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p>
<p><small>In the final step, the 3&rsquo; ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p>
<p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p>
</div>
</li>
</ul>
<p></p>
<h3>Reliable detection of enrichments in ChIP-seq</h3>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>&reg;</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p>
<p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>',
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			'format' => '12 rxns',
			'catalog_number' => 'C05010012',
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			'type' => 'FRE',
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			'meta_keywords' => '',
			'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)',
			'modified' => '2025-02-12 13:11:38',
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				[maximum depth reached]
			),
			(int) 140 => array(
				[maximum depth reached]
			),
			(int) 141 => array(
				[maximum depth reached]
			),
			(int) 142 => array(
				[maximum depth reached]
			),
			(int) 143 => array(
				[maximum depth reached]
			),
			(int) 144 => array(
				[maximum depth reached]
			),
			(int) 145 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			)
		),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	),
	(int) 1 => array()
)
$product = array()
CustomErrorHandler::handleError() - APP/Lib/CustomErrorHandler.php, line 9
include - APP/View/Pages/portal/shopping-list.ctp, line 1451
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
PagesController::display() - APP/Controller/PagesController.php, line 150
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118