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IGF2BP1 polyclonal antibody
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| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C. |
| Storage Buffer | PBS containing 50% glycerol, does not contain a preservative. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP | 15 µg per 107 cells | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
| IP | 5 µg per 2.5x106 cells | Fig 3 |
- Validation data


Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1
Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1
Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1
Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP1 (Cat. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above. - 应用
- 文档
IGF2BP1 polyclonal antibody datasheet
Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Bin...Download - 出版物

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(Notice): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context$errorType = self::getErrorType($code);self::logToDatabase( $errorType, $description, $file, $line);return parent::handleError( $errorType, $description, $file, $line, $context);$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410349', 'old_catalog_number' => '', 'sf_code' => 'C15410349-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '390', 'price_USD' => '390', 'price_GBP' => '345', 'price_JPY' => '63900', 'price_CNY' => '', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 19, 2018', 'slug' => 'igf2bp1-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'modified' => '2022-01-05 15:45:26', 'created' => '2018-06-28 14:36:19', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:23:01', 'created' => '2018-06-27 15:23:01', 'select_label' => '532 - IGF2BP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'fromEmail' => 'dev.diagenode.com' ) $language = 'cn' $meta_keywords = '' $meta_description = 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1' $meta_title = 'IGF2BP1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'modified' => '2022-01-05 15:45:26', 'created' => '2018-06-28 14:36:19', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410349', 'old_catalog_number' => '', 'sf_code' => 'C15410349-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '390', 'price_USD' => '390', 'price_GBP' => '345', 'price_JPY' => '63900', 'price_CNY' => '', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 19, 2018', 'slug' => 'igf2bp1-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'modified' => '2022-01-05 15:45:26', 'created' => '2018-06-28 14:36:19', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'fromEmail' => 'dev.diagenode.com' ) $language = 'cn' $meta_keywords = '' $meta_description = 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1' $meta_title = 'IGF2BP1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'modified' => '2022-01-05 15:45:26', 'created' => '2018-06-28 14:36:19', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:23:01', 'created' => '2018-06-27 15:23:01', 'select_label' => '532 - IGF2BP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410349', 'old_catalog_number' => '', 'sf_code' => 'C15410349-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '390', 'price_USD' => '390', 'price_GBP' => '345', 'price_JPY' => '63900', 'price_CNY' => '', 'price_AUD' => '975', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 19, 2018', 'slug' => 'igf2bp1-polyclonal-antibody-100-ug', 'meta_title' => 'IGF2BP1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'modified' => '2022-01-05 15:45:26', 'created' => '2018-06-28 14:36:19', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'fromEmail' => 'dev.diagenode.com' ) $language = 'cn' $meta_keywords = '' $meta_description = 'IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1) Polyclonal Antibody validated in IP, RIP and WB. Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1' $meta_title = 'IGF2BP1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2976', 'antibody_id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p> <p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. 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Batch-specific data available on the website. Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1', 'modified' => '2022-01-05 15:45:26', 'created' => '2018-06-28 14:36:19', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => '532', 'name' => 'IGF2BP1 polyclonal antibody', 'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>5 µg per 2.5x10<sup>6</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'igf2bp1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-06-27 15:23:01', 'created' => '2018-06-27 15:23:01', 'select_label' => '532 - IGF2BP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. 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