iDeal CUT&Tag kit for Histones MANUAL | Download |
Antibody package for CUT&Tag (anti-rabbit) DATASHEET | Download |
Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.
The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:
The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.
If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).
Check out:
Antibody Package for CUT&Tag (anti-rabbit) SDS GB en | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS US en | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS JP ja | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS DE de | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS BE nl | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS FR fr | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS BE fr | Download |
Antibody Package for CUT&Tag (anti-rabbit) SDS ES es | Download |
![]() How to properly cite our product/service in your workWe strongly recommend using this: Antibody package for CUT&Tag (anti-rabbit) (Hologic Diagenode Cat# C01070022). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12 |
RNA sequestration in P-bodies sustains myeloid leukaemia |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'product' => array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' ) $language = 'cn' $meta_keywords = '' $meta_description = '' $meta_title = 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022' $product = array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ) ), 'Group' => array( 'Group' => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ), 'Master' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '142', 'position' => '4', 'parent_id' => '13', 'name' => 'CUT&Tag', 'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p> <br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a> <div id="v5" class="content"> <p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p> <img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div> <h2>Products for CUT&Tag assay</h2> <h3 class="diacol">Complete solutions</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">Fusion protein</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li> </ul> <h3 class="diacol">CUT&Tag grade antibodies</h3> <ul class="nobullet"> <li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li> <li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li> <li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li> </ul> <h3 class="diacol">Positive & Negative CUT&Tag control</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">DNA purification</h3> <p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p> <h3 class="diacol">Sequencing indexes</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li> </ul> </li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'cutandtag', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ', 'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments', 'modified' => '2024-03-07 17:14:34', 'created' => '2020-04-28 15:07:36', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1160', 'name' => 'iDeal CUT&Tag kit for Histones', 'description' => '', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf', 'slug' => 'ideal-cut-and-tag-kit-for-histones-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2023-03-16 10:43:17', 'created' => '2022-06-29 15:43:39', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '5134', 'name' => 'Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12', 'authors' => 'Troester, Selina et al.', 'description' => '<div class="abstract" id="abstract"> <div class="abstract-content selected" id="eng-abstract"> <p style="text-align: justify;">Nucleoporin 98 (NUP98) fusion oncoproteins are strong drivers of pediatric acute myeloid leukemia (AML) with poor prognosis. Here we show that NUP98 fusion-expressing AML harbors an epigenetic signature that is characterized by increased accessibility of hematopoietic stem cell genes and enrichment of activating histone marks. We employ an AML model for ligand-induced degradation of the NUP98::KDM5A fusion oncoprotein to identify epigenetic programs and transcriptional targets that are directly regulated by NUP98::KDM5A through CUT&Tag and nascent RNA-seq. Orthogonal genome-wide CRISPR/Cas9 screening identifies 12 direct NUP98::KDM5A target genes, which are essential for AML cell growth. Among these, we validate cyclin-dependent kinase 12 (CDK12) as a druggable vulnerability in NUP98::KDM5A-expressing AML. In line with its role in the transcription of DNA damage repair genes, small-molecule-mediated CDK12 inactivation causes increased DNA damage, leading to AML cell death. Altogether, we show that NUP98::KDM5A directly regulates a core set of essential target genes and reveal CDK12 as an actionable vulnerability in AML with oncogenic NUP98 fusions.</p> </div> </div>', 'date' => '2025-05-19', 'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/40389480/', 'doi' => '10.1038/s41467-025-59930-9', 'modified' => '2025-06-06 15:59:08', 'created' => '2025-06-06 15:59:08', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2334', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS GB en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2336', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS US en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-US-en-1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2335', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-JP-ja-1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2331', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS DE de', 'language' => 'de', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-DE-de-1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2330', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2333', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2329', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) ) $pro = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( 'id' => '3253', 'product_id' => '3222', 'document_id' => '1163' ) ) $sds = array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( 'id' => '4027', 'product_id' => '3222', 'safety_sheet_id' => '2332' ) ) $publication = array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( 'id' => '8080', 'product_id' => '3222', 'publication_id' => '5090' ) ) $externalLink = ' <a href="https://www.nature.com/articles/s41556-024-01489-6" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'product' => array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' ) $language = 'cn' $meta_keywords = '' $meta_description = '' $meta_title = 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022' $product = array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ) ), 'Group' => array( 'Group' => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ), 'Master' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '142', 'position' => '4', 'parent_id' => '13', 'name' => 'CUT&Tag', 'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p> <br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a> <div id="v5" class="content"> <p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p> <img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div> <h2>Products for CUT&Tag assay</h2> <h3 class="diacol">Complete solutions</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">Fusion protein</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li> </ul> <h3 class="diacol">CUT&Tag grade antibodies</h3> <ul class="nobullet"> <li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li> <li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li> <li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li> </ul> <h3 class="diacol">Positive & Negative CUT&Tag control</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">DNA purification</h3> <p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p> <h3 class="diacol">Sequencing indexes</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li> </ul> </li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'cutandtag', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ', 'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments', 'modified' => '2024-03-07 17:14:34', 'created' => '2020-04-28 15:07:36', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1160', 'name' => 'iDeal CUT&Tag kit for Histones', 'description' => '', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf', 'slug' => 'ideal-cut-and-tag-kit-for-histones-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2023-03-16 10:43:17', 'created' => '2022-06-29 15:43:39', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '5134', 'name' => 'Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12', 'authors' => 'Troester, Selina et al.', 'description' => '<div class="abstract" id="abstract"> <div class="abstract-content selected" id="eng-abstract"> <p style="text-align: justify;">Nucleoporin 98 (NUP98) fusion oncoproteins are strong drivers of pediatric acute myeloid leukemia (AML) with poor prognosis. Here we show that NUP98 fusion-expressing AML harbors an epigenetic signature that is characterized by increased accessibility of hematopoietic stem cell genes and enrichment of activating histone marks. We employ an AML model for ligand-induced degradation of the NUP98::KDM5A fusion oncoprotein to identify epigenetic programs and transcriptional targets that are directly regulated by NUP98::KDM5A through CUT&Tag and nascent RNA-seq. Orthogonal genome-wide CRISPR/Cas9 screening identifies 12 direct NUP98::KDM5A target genes, which are essential for AML cell growth. Among these, we validate cyclin-dependent kinase 12 (CDK12) as a druggable vulnerability in NUP98::KDM5A-expressing AML. In line with its role in the transcription of DNA damage repair genes, small-molecule-mediated CDK12 inactivation causes increased DNA damage, leading to AML cell death. Altogether, we show that NUP98::KDM5A directly regulates a core set of essential target genes and reveal CDK12 as an actionable vulnerability in AML with oncogenic NUP98 fusions.</p> </div> </div>', 'date' => '2025-05-19', 'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/40389480/', 'doi' => '10.1038/s41467-025-59930-9', 'modified' => '2025-06-06 15:59:08', 'created' => '2025-06-06 15:59:08', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2334', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS GB en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2336', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS US en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-US-en-1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2335', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-JP-ja-1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2331', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS DE de', 'language' => 'de', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-DE-de-1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2330', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2333', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2329', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) ) $pro = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( 'id' => '3253', 'product_id' => '3222', 'document_id' => '1163' ) ) $sds = array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( 'id' => '4027', 'product_id' => '3222', 'safety_sheet_id' => '2332' ) ) $publication = array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( 'id' => '8080', 'product_id' => '3222', 'publication_id' => '5090' ) ) $externalLink = ' <a href="https://www.nature.com/articles/s41556-024-01489-6" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'product' => array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' ) $language = 'cn' $meta_keywords = '' $meta_description = '' $meta_title = 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022' $product = array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ) ), 'Group' => array( 'Group' => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ), 'Master' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '142', 'position' => '4', 'parent_id' => '13', 'name' => 'CUT&Tag', 'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p> <br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a> <div id="v5" class="content"> <p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p> <img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div> <h2>Products for CUT&Tag assay</h2> <h3 class="diacol">Complete solutions</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">Fusion protein</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li> </ul> <h3 class="diacol">CUT&Tag grade antibodies</h3> <ul class="nobullet"> <li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li> <li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li> <li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li> </ul> <h3 class="diacol">Positive & Negative CUT&Tag control</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">DNA purification</h3> <p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p> <h3 class="diacol">Sequencing indexes</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li> </ul> </li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'cutandtag', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ', 'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments', 'modified' => '2024-03-07 17:14:34', 'created' => '2020-04-28 15:07:36', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1160', 'name' => 'iDeal CUT&Tag kit for Histones', 'description' => '', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf', 'slug' => 'ideal-cut-and-tag-kit-for-histones-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2023-03-16 10:43:17', 'created' => '2022-06-29 15:43:39', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '5134', 'name' => 'Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12', 'authors' => 'Troester, Selina et al.', 'description' => '<div class="abstract" id="abstract"> <div class="abstract-content selected" id="eng-abstract"> <p style="text-align: justify;">Nucleoporin 98 (NUP98) fusion oncoproteins are strong drivers of pediatric acute myeloid leukemia (AML) with poor prognosis. Here we show that NUP98 fusion-expressing AML harbors an epigenetic signature that is characterized by increased accessibility of hematopoietic stem cell genes and enrichment of activating histone marks. We employ an AML model for ligand-induced degradation of the NUP98::KDM5A fusion oncoprotein to identify epigenetic programs and transcriptional targets that are directly regulated by NUP98::KDM5A through CUT&Tag and nascent RNA-seq. Orthogonal genome-wide CRISPR/Cas9 screening identifies 12 direct NUP98::KDM5A target genes, which are essential for AML cell growth. Among these, we validate cyclin-dependent kinase 12 (CDK12) as a druggable vulnerability in NUP98::KDM5A-expressing AML. In line with its role in the transcription of DNA damage repair genes, small-molecule-mediated CDK12 inactivation causes increased DNA damage, leading to AML cell death. Altogether, we show that NUP98::KDM5A directly regulates a core set of essential target genes and reveal CDK12 as an actionable vulnerability in AML with oncogenic NUP98 fusions.</p> </div> </div>', 'date' => '2025-05-19', 'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/40389480/', 'doi' => '10.1038/s41467-025-59930-9', 'modified' => '2025-06-06 15:59:08', 'created' => '2025-06-06 15:59:08', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2334', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS GB en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2336', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS US en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-US-en-1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2335', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-JP-ja-1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2331', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS DE de', 'language' => 'de', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-DE-de-1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2330', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2333', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2329', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) ) $pro = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( 'id' => '3253', 'product_id' => '3222', 'document_id' => '1163' ) ) $sds = array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( 'id' => '4027', 'product_id' => '3222', 'safety_sheet_id' => '2332' ) ) $publication = array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( 'id' => '8080', 'product_id' => '3222', 'publication_id' => '5090' ) ) $externalLink = ' <a href="https://www.nature.com/articles/s41556-024-01489-6" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/var/www/dev.diagenode.com/app/View/Products/view.ctp' $dataForView = array( 'language' => 'cn', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'product' => array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' ) $language = 'cn' $meta_keywords = '' $meta_description = '' $meta_title = 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022' $product = array( 'Product' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p><p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p><ul><li>Anti-rabbit secondary antibody</li><li>Rabbit H3K27me3 positive control antibody</li><li>Rabbit negative IgG control</li><li>TSH2B primer pair (human)</li><li>GAPDH TSS primer pair (human)</li></ul><p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p><p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).</p><p><strong>Check out:</strong></p><ul><li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li><li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li></ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00', 'locale' => 'zho' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ) ), 'Group' => array( 'Group' => array( 'id' => '366', 'name' => 'CUT&Tag Anti-rabbit', 'product_id' => '3222', 'modified' => '2024-01-11 11:21:58', 'created' => '2024-01-11 11:21:58' ), 'Master' => array( 'id' => '3222', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '24 rxns', 'catalog_number' => 'C01070022', 'old_catalog_number' => '', 'sf_code' => 'C01070022-', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '165', 'price_USD' => '185', 'price_GBP' => '150', 'price_JPY' => '27035', 'price_CNY' => '', 'price_AUD' => '462', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-24', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode C01070022', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:22:45', 'created' => '2022-06-29 12:14:00' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '142', 'position' => '4', 'parent_id' => '13', 'name' => 'CUT&Tag', 'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p> <br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a> <div id="v5" class="content"> <p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p> <img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div> <h2>Products for CUT&Tag assay</h2> <h3 class="diacol">Complete solutions</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">Fusion protein</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li> </ul> <h3 class="diacol">CUT&Tag grade antibodies</h3> <ul class="nobullet"> <li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li> <li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li> <li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li> </ul> <h3 class="diacol">Positive & Negative CUT&Tag control</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li> </ul> <h3 class="diacol">DNA purification</h3> <p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p> <h3 class="diacol">Sequencing indexes</h3> <ul class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li> </ul> </li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'cutandtag', 'cookies_tag_id' => null, 'meta_keywords' => '', 'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ', 'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments', 'modified' => '2024-03-07 17:14:34', 'created' => '2020-04-28 15:07:36', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1160', 'name' => 'iDeal CUT&Tag kit for Histones', 'description' => '', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf', 'slug' => 'ideal-cut-and-tag-kit-for-histones-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2023-03-16 10:43:17', 'created' => '2022-06-29 15:43:39', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '5134', 'name' => 'Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12', 'authors' => 'Troester, Selina et al.', 'description' => '<div class="abstract" id="abstract"> <div class="abstract-content selected" id="eng-abstract"> <p style="text-align: justify;">Nucleoporin 98 (NUP98) fusion oncoproteins are strong drivers of pediatric acute myeloid leukemia (AML) with poor prognosis. Here we show that NUP98 fusion-expressing AML harbors an epigenetic signature that is characterized by increased accessibility of hematopoietic stem cell genes and enrichment of activating histone marks. We employ an AML model for ligand-induced degradation of the NUP98::KDM5A fusion oncoprotein to identify epigenetic programs and transcriptional targets that are directly regulated by NUP98::KDM5A through CUT&Tag and nascent RNA-seq. Orthogonal genome-wide CRISPR/Cas9 screening identifies 12 direct NUP98::KDM5A target genes, which are essential for AML cell growth. Among these, we validate cyclin-dependent kinase 12 (CDK12) as a druggable vulnerability in NUP98::KDM5A-expressing AML. In line with its role in the transcription of DNA damage repair genes, small-molecule-mediated CDK12 inactivation causes increased DNA damage, leading to AML cell death. Altogether, we show that NUP98::KDM5A directly regulates a core set of essential target genes and reveal CDK12 as an actionable vulnerability in AML with oncogenic NUP98 fusions.</p> </div> </div>', 'date' => '2025-05-19', 'pmid' => 'https://pubmed.ncbi.nlm.nih.gov/40389480/', 'doi' => '10.1038/s41467-025-59930-9', 'modified' => '2025-06-06 15:59:08', 'created' => '2025-06-06 15:59:08', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2334', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS GB en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2336', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS US en', 'language' => 'en', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-US-en-1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2335', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-JP-ja-1_0.pdf', 'countries' => 'JP', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2331', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS DE de', 'language' => 'de', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-DE-de-1_0.pdf', 'countries' => 'DE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '2330', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2333', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2329', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://dev.diagenode.com/cn/p/antibody-package-cut-and-tag-antirabbit-24' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) ) $pro = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3236', 'antibody_id' => null, 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.</p> <p>The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:</p> <ul> <li>Anti-rabbit secondary antibody</li> <li>Rabbit H3K27me3 positive control antibody</li> <li>Rabbit negative IgG control</li> <li>TSH2B primer pair (human)</li> <li>GAPDH TSS primer pair (human)</li> </ul> <p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p> <p>If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&Tag (anti-mouse)</a>.</p> <p><strong>Check out:</strong></p> <ul> <li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a></li> <li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade antibodies</a></li> </ul>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '8 rxns', 'catalog_number' => 'C01070026', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '80', 'price_USD' => '90', 'price_GBP' => '70', 'price_JPY' => '13110', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'antibody-package-cut-and-tag-antirabbit-8', 'meta_title' => 'Antibody package for CUT&Tag (anti-rabbit) | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-01-11 11:15:13', 'created' => '2023-12-20 14:22:03', 'ProductsGroup' => array( 'id' => '425', 'product_id' => '3236', 'group_id' => '366' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '1163', 'name' => 'Antibody package for CUT&Tag (anti-rabbit)', 'description' => '', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf', 'slug' => 'antibody-package-for-cutandtag-anti-rabbit', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-07 11:10:51', 'created' => '2022-10-07 11:10:51', 'ProductsDocument' => array( 'id' => '3253', 'product_id' => '3222', 'document_id' => '1163' ) ) $sds = array( 'id' => '2332', 'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-29 14:35:38', 'created' => '2022-08-29 14:35:38', 'ProductsSafetySheet' => array( 'id' => '4027', 'product_id' => '3222', 'safety_sheet_id' => '2332' ) ) $publication = array( 'id' => '5090', 'name' => 'RNA sequestration in P-bodies sustains myeloid leukaemia', 'authors' => 'Srikanth Kodali et al.', 'description' => '<p><span>Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.</span></p>', 'date' => '2024-08-21', 'pmid' => 'https://www.nature.com/articles/s41556-024-01489-6', 'doi' => 'https://doi.org/10.1038/s41556-024-01489-6', 'modified' => '2025-03-27 09:34:07', 'created' => '2025-03-27 09:34:07', 'ProductsPublication' => array( 'id' => '8080', 'product_id' => '3222', 'publication_id' => '5090' ) ) $externalLink = ' <a href="https://www.nature.com/articles/s41556-024-01489-6" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.