DNA Methylation
Methylated DNA immunoprecipitation
The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.
How it works
In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.Overview
针对 Methylated DNA immunoprecipitation 的推荐产品
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010031 |
hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto... |
16 rxns | $690.00 | |
| C02010021 |
MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo... |
48 rxns (IP) | $770.00 | |
| C02010041 |
MagMeDIP kit (Coming soon) Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain r... |
10 rxns | ||
| C30010014 |
2 ml microtubes for SX-8G IP-Star® Compact These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System. |
100 pc | $50.00 | |
| C15410205 |
5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit) Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH. |
50 μg | $390.00 | |
| C15200006-100 |
5-methylcytosine (5-mC) Antibody - cl. b Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine. |
100 µg | $465.00 | |
| C15200081-100 |
5-methylcytosine (5-mC) Antibody - clone 33D3 Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine (33D3 clone). |
100 µg | $590.00 | |
| C02010034 |
Auto hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy... |
16 rxns | $690.00 | |
| C02010014 |
Auto MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy... |
48 rxns | ||
| C02040018 |
DNA hydroxymethylation control package The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be ad... |
48 rxns | $155.00 | |
| C02040012 |
DNA Methylation control package The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your... |
40 rxns | $310.00 | |
| C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes... |
48 rxns | $285.00 | |
| C15400001-15 |
Mouse IgG Antibodies - Chip The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses ... |
15 µg/15 µl | $75.00 | |
| C15410206 |
Rabbit IgG The negative Ctrl IgG from rabbit has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses... |
250 µg/250 µl | $85.00 | |
| C15420001 |
Rat IgG The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in ser... |
25 µg/ 25 µl | $95.00 |