Chromatin shearing
Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.
Panel A, 10 µl volume: Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). Panel B, 100 µl volume: Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). Panel C, 300 µl volume: Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).
It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.
| Chromatin Shearing Kit Low SDS (for Histone) | Chromatin Shearing Kit Low SDS (for TF) | Chromatin Shearing Kit High SDS | Chromatin Shearing Kit (for Plant) | |
|
SDS concentration |
< 0.1% |
0.2% |
1% |
0.5% |
|
Nuclei isolation |
Yes |
Yes |
No |
Yes |
|
Allows for shearing of... cells/tissue |
100 million cells |
100 million cells |
100 million cells |
up to 25 g of tissue |
|
Corresponding to shearing buffers from |
Table comes from our Guide for successful chromatin preparation using the Bioruptor® Pico
针对 Chromatin shearing 的推荐产品
-
B012000410.1 ml tube holder & tube adaptors for Biorupto...
-
B0120001210 ml tube holder for Bioruptor® Standard & Plus
相关方案
| Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c... | 下载 |
| Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro... | 下载 |
| ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing. | 下载 |