Step by step workflow |
Optimal solution from Diagenode |
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1. Crosslink to bind proteins to DNA  |
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2. Shear DNA  |
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3. Immunoprecipitate with specific antibody  |
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4. Reverse crosslinks and purify  |
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5. ChIP-qPCR or ChIP-seq library preparation |
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Chromatin studies
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci. ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites.
The ChIP workflow
Products for chromatin study
Crosslinking
Efficient solution for protein-protein fixation. Read more
Chromatin shearing
Perfectly sheared chromatin is critical for ChIP success.
Read more about reagent optimization.
Read more about Bioruptor sonication.
Chromatin immunoprecipitation
Immunoprecipitation solutions for histone and transcription factor ChIP-qPCR and ChIP-seq for low inputs, plants, and animals including automated solutions. Read more
ChIPmentation
ChIPmentation, an exclusive technology, is an end-to-end ChIP-seq solution for low and difficult inputs. Read more
ChIP and ChIP-seq antibodies
ChIP-grade antibodies are essential for success. Learn more
Primer pairs
Highly specific primer pairs for the amplification of the specific genomic regions. Read more
DNA purification
Read more about solutions for DNA purification
Library preparation for ChIP-seq
Optimized solutions for the library preparation from low DNA input. Read more
ChIP and ChIP-seq automation
Reproducibility, optimization simplicity, no variability. Learn more
Chromatin assembly products
Study the nucleosome environment. Learn more
Chromatin resources
Posters
- Understanding our antibody QC
- High quality ChIP antibodies
- ChIP with only 10,000 cells
- High resolution ChIP-seq using automation
- ChIP-seq bioinformatics
Application notes
- Simple semi-automaton for easy and inexpensive ChIP
- Performing ChIP-seq on human tumor tissue
- Plant ChIP-seq – a successful method
- Best workflow practices for low input ChIP
- Optimize the selection of guide RNA by ChIP to keep CRISPR on-target
Publications related to ChIP
- Corticosteroid receptors adopt distinct cyclical transcriptional signatures
- Pro-inflammatory cytokine and high doses of ionizing radiation have similar effects on the expression of NF-kappaB-dependent genes
- Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile
Brochures
产品
| 目录号 | 产品 | 格式 | 价格 | |
|---|---|---|---|---|
| C01080002 |
ATAC-seq kit |
24 rxns | $1,745.00 |
Chromatin shearing
Chromatin immunoprecipitation
ChIPmentation™
| 目录号 | 产品 | 格式 | 价格 | |
|---|---|---|---|---|
| C01011011 |
µChIPmentation for Histones The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries. T... |
24 rxns | $2,400.00 | |
| C01011032 |
SI for Tagmented libraries The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries ar... |
24 SI | $190.00 | |
| C01011000 |
Auto ChIPmentation Kit for Histones Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ... |
24 rxns | ||
| C01011010 |
ChIPmentation Kit for Histones Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ... |
24 rxns | ||
| C01011030 |
TAG Kit for ChIPmentation Contact us Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fra... |
24 rxns | $1,050.00 |
